Mouse TIM-2 Antibody (222919)

R&D Systems | Catalog # MAB1885

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 222919
View all TIM-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TIM‑2
His22-Asn229
Accession # NP_599010

Specificity

Detects human TIM-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human TIM-1, recombinant mouse (rm) TIM-1 or rmTIM-3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse TIM-2 Antibody (222919)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse TIM‑2

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIM-2

TIM-2 is a type I transmembrane protein that has an immunoglobulin variable region-like domain followed by a mucin-like domain in its extracellular region. It is selectively expressed on activated T cells and binds to Semaphorin 4A.

Long Name

T Cell Immunoglobulin Mucin-2

Alternate Names

TIM2

Entrez Gene IDs

171284 (Mouse)

Gene Symbol

TIMD2

UniProt

NP_599010

Additional TIM-2 Products

Product Documents for Mouse TIM-2 Antibody (222919)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TIM-2 Antibody (222919)

For research use only

Citations for Mouse TIM-2 Antibody (222919)

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FAQs

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