Mouse TIM-4 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF2826T Datasheet / COA / SDS

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AF2826T-100UG

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All TIM-4 Antibodies

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Mouse TIM-4 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TIM-4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with recombinant human (rh) TIM-1, recombinant mouse (rm) TIM-2, rmTIM-3, rhTIM-4, rmTIM-5, and rmTIM-6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TIM-4
Ala22-Thr279
Accession # Q6U7R4

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TIM-4

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1 - 3). The mouse TIM-4 cDNA encodes a 343 amino acid (aa) precursor that includes a 22 aa signal sequence, a 257 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1, -2, or -3. Within the ECD, mouse TIM-4 shares 27 - 33% aa sequence identity with mouse TIM-1, -2, and -3. The ECD of mouse TIM-4 shares 45% aa sequence identity with that of human and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for hepatitis A virus and has been implicated in the development of asthma (5 ‑ 7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4⁺ T cells (5, 8). The interaction of TIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells (5).

Long Name

T Cell Immunoglobulin Mucin-4

Entrez Gene IDs

91937 (Human); 276891 (Mouse); 102140672 (Cynomolgus Monkey)

Alternate Names

FLJ27515; Smuckler; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell membrane protein 4; TIM4; TIM-4; TIMD4; TIMD-4