Mouse TREM-3 Antibody (295502)

R&D Systems | Catalog # MAB2224

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 295502
View all TREM-3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TREM-3
Met1-Pro135 (predicted)
Accession # NP_067382

Specificity

Detects mouse TREM-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) TREM-1 or rmTREM-2b is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse TREM-3 Antibody (295502)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse TREM‑3

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TREM-3

TREM-3 is a type I transmembrane protein possessing a single extracellular Ig domain. It associates with the signal adapter molecule, DAP12, and delivers an activating signal. TREM-3 is expressed in macrophage cell lines and at low levels in T cell lines, but not in NK, B cell, or mast cell lines.

Long Name

Triggering Receptor Expressed on Myeloid Cells 3

Alternate Names

TREM3

Entrez Gene IDs

58218 (Mouse)

Gene Symbol

TREM3

UniProt

NP_067382

Additional TREM-3 Products

Product Documents for Mouse TREM-3 Antibody (295502)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TREM-3 Antibody (295502)

For research use only

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FAQs

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