Mouse TREML4/TLT-4 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF5500G Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5500G-100UG

Save 15% on Select RUO Reagents. See Details.

All TREML4/TLT-4 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TREML4/TLT-4 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TREML4/TLT‑4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) TREM1, rmTREM2b, and rmTREM3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TREML4/TLTâ€‘4
Ser25-Val195
Accession # NP_001029094

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TREML4/TLT-4

TREML4 (Triggering receptor expressed on myeloid cells-like 4; also TLT4) is a 53-55 kDa member of the Ig superfamily. It is likely expressed by leukocytes, and based on its structure, may act as an activating receptor. Mature mouse TREML4 is a type I transmembrane (TM) glycoprotein that is 235 amino acids (aa) in length. It contains a 171 aa extracellular region (aa 29-199) with a V-type Ig-like domain (aa 29-122); the TM segment contains one Lys residue. There is one splice variant that shows a deletion of aa 21-24, a segment that lies in the putative signal sequence. Rodent and human TREML4 are highly divergent, and thus over aa 25-195, mouse TREML4 shares 78% aa sequence identity with rat TREML4, but only 52% aa sequence identity with human TREML4.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 4

Entrez Gene IDs

285852 (Human); 224840 (Mouse); 680862 (Rat)

Alternate Names

TLT4; TREML4