Mouse Wnt-10b Alexa Fluor® 488-conjugated Antibody

Catalog #: AF2110G Datasheet / COA / SDS

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AF2110G-100UG

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Mouse Wnt-10b Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Wnt-10b in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse (rm) Wnt-3a, rmWnt-4, rmWnt-5a, and rmWnt-8a is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Wnt-10b peptide
Leu42-Glu82, Arg263-Arg332
Accession # P48614

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Wnt-10b

Wnt-10b (also known as Wnt-12) is a 45 kDa glycoprotein involved in fetal limb bud development and adult hematopoiesis. Mouse Wnt-10b exists as two isoforms: a 361 amino acid (aa) long form and a 265 aa short form that lacks a 95 aa internal sequence. Wnt-10b is less than 60% aa identical to other Wnts, but mouse to human, Wnt-10b shows almost complete aa sequence identity at > 97%.

Long Name

Wingless-type MMTV Integration Site Family, Member 10B

Entrez Gene IDs

7480 (Human); 22410 (Mouse)

Alternate Names

protein Wnt-10b; Protein Wnt-12; SHFM6WNT-10B protein; wingless-type MMTV integration site family, member 10B; Wnt10b; Wnt-10b; WNT12; Wnt-12