Mouse Wnt-8b Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB33671X Datasheet / COA / SDS

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FAB33671X-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Wnt-8b Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Wnt-8b in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Wnt-2, -7a, -7b,
-9a, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, or -9b is observed.

Source

Monoclonal Rat IgG2a Clone # 341502

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse Wnt‑8b
Trp22-Glu52, Leu195-Arg269
Accession # Q9WUD6

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Wnt-8b

Mouse Wnt-8b is a 40-50 kDa secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329 aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98%, and 97% identical to mature rat, canine, and human Wnt-8b, respectively.

Long Name

Wingless-type MMTV Integration Site Family, Member 8b

Entrez Gene IDs

7479 (Human); 22423 (Mouse); 293990 (Rat)

Alternate Names

protein Wnt-8b; wingless-type MMTV integration site family, member 8B; Wnt8b; Wnt-8b