MT-ND1 Antibody (JE34-93)

Novus Biologicals | Catalog # NBP3-32613

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE34-93 expressed in HEK293
View all MT-ND1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human MT-ND1 aa 23-67 / 318. (Uniprot: P03886)

Localization

Mitochondrion inner membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MT-ND1 Antibody (JE34-93)

MT-ND1 Antibody (JE34-93) Western Blot: MT-ND1 Antibody (JE34-93) [NBP3-32613]

Western Blot: MT-ND1 Antibody (JE34-93) [NBP3-32613]

Western blot analysis on different lysates with NBP3-32613 at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 36 kDa Observed band size: 37 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32613 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
MT-ND1 Antibody (JE34-93) Western Blot: MT-ND1 Antibody (JE34-93) [NBP3-32613]

Western Blot: MT-ND1 Antibody (JE34-93) [NBP3-32613]

Western blot analysis on different lysates with NBP3-32613 at 1/1,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Rat skeletal muscle tissue lysate Lane 3: Mouse smooth muscle tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 37 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32613 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

Applications for MT-ND1 Antibody (JE34-93)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MT-ND1

NADH:ubiquinone oxidoreductase (complex I) is an extremely complicated multiprotein complex located in the inner mitochondrial membrane. Human complex I is important for energy metabolism because its main function is to transport electrons from NADH to ubiquinone, which is accompanied by translocation of protons from the mitochondrial matrix to the intermembrane space. Human complex I appears to consist of 41 subunits. A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7),while the remainder are nuclear encoded and imported from the cytoplasm. NADH dehydrogenase subunit 1 (ND1) binds rotenone and rotenone analogs and might be involved in electron transfer to ubiquinone. Mutations in the ND1 gene may be implicated in several disorders, including Leber hereditary optic neuropathy, Alzheimer's disease and Parkinson disease.

Alternate Names

EC 1.6.5.3, mitochondrially encoded NADH dehydrogenase 1, MTND1, NAD1, NADH dehydrogenase subunit 1, NADH dehydrogenase, subunit 1 (complex I), NADH1, ND1NADH dehydrogenase 1

Gene Symbol

ND1

Additional MT-ND1 Products

Product Documents for MT-ND1 Antibody (JE34-93)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MT-ND1 Antibody (JE34-93)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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