MTA3 Antibody (OTI2B8)

Novus Biologicals | Catalog # NBP3-44819

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2B8
View all MTA3 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-259 of human MTA3 (NP_065795) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MTA3 Antibody (OTI2B8)

MTA3 Antibody (OTI2B8) Western Blot;MTA3 Antibody (OTI2B8)[NBP3-44819]-

Western Blot;MTA3 Antibody (OTI2B8)[NBP3-44819]-

Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-MTA3 monoclonal antibody (1:500).
MTA3 Antibody (OTI2B8) Western Blot;MTA3 Antibody (OTI2B8)[NBP3-44819]-

Western Blot;MTA3 Antibody (OTI2B8)[NBP3-44819]-

HEK293T cells were transfected with the pCMV6-ENTRY control ( Left lane) or pCMV6-ENTRY MTA3 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTA3(1:2000). Positive lysates

Applications for MTA3 Antibody (OTI2B8)

Application
Recommended Usage

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

>95%

Reconstitution

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized powder ( 1X PBS, pH 7.3,)

Preservative

8% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MTA3

MTA3 plays a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels. MTA3 gene encodes for two forms, a 515 amino acids and a longer protein of 594 amino acids, called MTA3L.

Alternate Names

member 3, metastasis associated 1 family, member 3, metastasis-associated protein MTA3

Gene Symbol

MTA3

Additional MTA3 Products

Product Documents for MTA3 Antibody (OTI2B8)

Certificate of Analysis

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Product Specific Notices for MTA3 Antibody (OTI2B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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