MTT

Tocris Bioscience | Catalog # 5224

Reagent used in the measurement of cell proliferation
Tocris Bioscience

Key Product Details

Description

Reagent used in the measurement of cell proliferation
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Product Description

MTT is a reagent used in the measurement of cell proliferation.

Product Specifications for MTT

Molecular Weight

414.32

Formula

C18H16N5SBr

Storage

Store at +4°C

Purity

≥98%

Chemical Name

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide

CAS Number

298-93-1

PubChem ID

64965

InChI Key

AZKSAVLVSZKNRD-UHFFFAOYSA-M

SMILES

CC1=C(C)SC([N+]2=NC(C4=CC=CC=C4)=NN2C3=CC=CC=C3)=N1.[Br-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
PBS 5 13

Preparing Stock Solutions for MTT

The following data is based on the product molecular weight 414.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.13 mM 18.57 mL 92.83 mL 185.66 mL
0.65 mM 3.71 mL 18.57 mL 37.13 mL
1.3 mM 1.86 mL 9.28 mL 18.57 mL
6.5 mM 0.37 mL 1.86 mL 3.71 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 25 publications citing the usage of this product.

There are currently no references for this product.

Product Documents for MTT

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MTT

For research use only

Customer Reviews for MTT (3)

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  • MTT
    Name: Chi Ngo
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: ARPE: retinal pigmented epithelial cell line
    Verified Customer | Posted 09/06/2025
    I used MTT to measure cell viability of Retinal pigmented epithelial cell (ARPE) in response to hyperglycemia with/without hypothesized chemoprotective compounds.
    MTT 5224
  • Detection of apoptosis via cell proliferation assay MTT was carried out
    Name: Anonymous
    Species: Human
    Verified Customer | Posted 02/11/2019
    Detection of apoptosis via cell proliferation assay MTT was carried out.The 10 µl solution of MTT is added to one of the 96 well- plate and then incubate the plate for 4 hours. After incubation time the medium from wells was discarded and 100 µl of acid-propanol added to all well of the plate and incubated for 30 minutes at room temperature. Then the absorbance of each well were measured at 340 nm by plate reader.
    MTT 5224
  • MTT as a tool to estimate cell proliferation
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Cardiomyocytes
    Verified Customer | Posted 12/12/2018
    HL-1 cells were treated with 15d-PGJ2 (15 μM) for 0-12 h followed by incubation with MTT (0.5 mg/ml; dissolved in serum-free medium) for 30 min at 37°C. The converted insoluble dark purple coloured dye (formazan) was solubilized with 300 µl acidic isopropanol (0.04 M HCl in absolute isopropanol). Cell proliferation was assessed by measuring optical densities at emission and correction wavelengths of 570 and 630 nm, respectively, on a microtiter plate reader.
    MTT 5224

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