Q: Our company might be interested in Novus product PFK-1 Protein (NBP1-44494) (recombinant phosphofructokinase). Product page of Novus website does not indicate whether the protein is active or not. If the former, how this activity was tested? Could you please clarify on that?

A: This protein NBP1-44494 has not been tested for activity by the lab. As such we cannot guarantee if it is active or not and expect most likely it will not be active. Mainly this is intended as a control for Western Blot.

Recombinant Human Muscle Phosphofructokinase/PFKM/PFK-1 His Protein

Novus Biologicals | Catalog # NBP1-44494

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Product Documents

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all Muscle Phosphofructokinase/PFKM Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-780 of Human Muscle Phosphofructokinase/PFKM/PFK-1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTHEEHHAAK TLGIGKAIAV LTSGGDAQGM NAAVRAVVRV GIFTGARVFF VHEGYQGLVD GGDHIKEATW ESVSMMLQLG GTVIGSARCK DFREREGRLR AAYNLVKRGI TNLCVIGGDG SLTGADTFRS EWSDLLSDLQ KAGKITDEEA TKSSYLNIVG LVGSIDNDFC GTDMTIGTDS ALHRIMEIVD AITTTAQSHQ RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE EHLCRRLSET RTRGSRLNII IVAEGAIDKN GKPITSEDIK NLVVKRLGYD TRVTVLGHVQ RGGTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSG NQAVRLPLME CVQVTKDVTK AMDEKKFDEA LKLRGRSFMN NWEVYKLLAH VRPPVSKSGS HTVAVMNVGA PAAGMNAAVR STVRIGLIQG NRVLVVHDGF EGLAKGQIEE AGWSYVGGWT GQGGSKLGTK RTLPKKSFEQ ISANITKFNI QGLVIIGGFE AYTGGLELME GRKQFDELCI PFVVIPATVS NNVPGSDFSV GADTALNTIC TTCDRIKQSA AGTKRRVFII ETMGGYCGYL ATMAGLAAGA DAAYIFEEPF TIRDLQANVE HLVQKMKTTV KRGLVLRNEK CNENYTTDFI FNLYSEEGKG IFDSRKNVLG HMQQGGSPTP FDRNFATKMG AKAMNWMSGK IKESYRNGRI FANTPDSGCV LGMRKRALVF QPVAELKDQT DFEHRIPKEQ WWLKLRPILK ILAKYEIDLD TSDHAHLEHI TRKRSGEAAV

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

87.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Muscle Phosphofructokinase/PFKM/PFK-1 His Protein

SDS-PAGE: Recombinant Human Muscle Phosphofructokinase/PFKM/PFK-1 His Protein [NBP1-44494]

SDS-Page: Recombinant Human Muscle Phosphofructokinase/PFKM/PFK-1 Protein [NBP1-44494] - PFKM, 87.3 kDa (800aa), confirmed by MALDI-TOF with a purity of 80% by SDS - PAGE

Formulation, Preparation, and Storage

NBP1-44494

Formulation	20 mM Tris-HCl buffer (pH8.0), 5mM DTT, 0.2 M NaCl, and 20% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Muscle Phosphofructokinase/PFKM

PFKM is regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Recombinant human PFKM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Phosphofructokinase, Muscle

Alternate Names

GSD7, PFK-1, PFK-A, PFKM, PFKX, Phosphofructokinase-1, Phosphofructokinase-M, Phosphohexokinase

Gene Symbol

PFKM

Additional Muscle Phosphofructokinase/PFKM Products

Product Documents for Recombinant Human Muscle Phosphofructokinase/PFKM/PFK-1 His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Muscle Phosphofructokinase/PFKM/PFK-1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

Citations for Recombinant Human Muscle Phosphofructokinase/PFKM/PFK-1 His Protein

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