N OLLAS Vector

Name: N OLLAS Vector
Brand: Novus Biologicals
SKU: NBA1-07083
Price: 329 USD
Availability: InStock

Novus Biologicals | Catalog # NBA1-07083

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Product Specifications

Specificity

pCMV-N OLLAS Vector for the intracellular expression of N-terminally OLLAS-tagged protein.

Application Notes

Using the N-OLLAS vector the protein will be expressed in the cytoplasm. We recommend inserting your protein of interest into the vector in frame using the Hind III and Not I restriction sites shown on the vector map above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants.

Scientific Data Images for N OLLAS Vector

Western Blot: N OLLAS Vector [NBA1-07083]

Western Blot: N OLLAS Vector [NBA1-07083] - Western blot comparison of binding sensitivity of Novus Biologicals monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Other: N OLLAS Vector [NBA1-07083]

N OLLAS Vector [NBA1-07083] - Vector map of pCMV-N OLLAS

Formulation, Preparation, and Storage

Formulation

5 mM Tris pH 8.0

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: N OLLAS Vector

The pCMV-N-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with an N-terminal OLLAS tag. Using the N-OLLAS vector the protein will be expressed in the cytoplasm. We recommend inserting your protein of interest into the vector in frame using the Hind III and Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.

Alternate Names

OLLA-2 vector

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Product Specific Notices for N OLLAS Vector

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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