N6-methyladenosine (m6A) Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-18690
Loading...
Key Product Details
Species Reactivity
Human, Mouse
Applications
RNA immunoprecipitation, Dot Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Format
BSA Free
Loading...
Product Specifications
Immunogen
Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for N6-methyladenosine (m6A) Antibody - BSA Free
Dot Blot: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 4. Dot blot analysis using the antibody directed against N6-methyladenosine (m6A) To demonstrate the specificity of the antibody against N6-methyladenosine (m6A), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligos were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.
RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 1. RNA immunoprecipitation using the antibody directed against N6-methyladenosine (m6A) RNA Immunoprecipitation was performed on 40 ug HeLa total RNA spiked with 0.5 ug of an in vitro prepared transcript containing N6-methyladenosine (m6A) nucleotides, using 2 ug of the N6-methyladenosine (m6A) antibody. An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 3. RIP-seq results obtained with the antibody directed against N6-methyladenosine (m6A) RIP was performed with 2 ug of the antibody against N6-methyladenosine (m6A). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).
RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 2. RNA immunoprecipitation using the antibody directed against N6-methyladenosine (m6A) RNA Immunoprecipitation was performed on 40 ug HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 ug of antibody per RIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3 UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Immunoprecipitation: Rabbit N6-methyladenosine (m6A) pAb [NBP3-18690]
RNA immunoprecipitation (RIP) was performed using either IgG or m6A Antibody (NBP3-18690) (2 ug/IP) with 50 ug MDA-MB-231 total RNA, followed by qPCR with primers for PRSS23 gene. Image from a verified customer review.Applications for N6-methyladenosine (m6A) Antibody - BSA Free
Application
Recommended Usage
Dot Blot
1:400
RNA immunoprecipitation
1-2 ug/IP
Application Notes
Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 ug per IP.
Reviewed Applications
Read 1 review rated 5 using NBP3-18690 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide and 0.05% ProClin 300
Concentration
1.2 mg/ml
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: N6-methyladenosine (m6A)
Alternate Names
(-)-6-(Methylamino)purine riboside, N(6)-Methyladenosine, N-Methyladenosine
Additional N6-methyladenosine (m6A) Products
Product Documents for N6-methyladenosine (m6A) Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for N6-methyladenosine (m6A) Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for N6-methyladenosine (m6A) Antibody - BSA Free
Powered by Bioz
Customer Reviews for N6-methyladenosine (m6A) Antibody - BSA Free (1)
5 out of 5
1 Customer Rating
Have you used N6-methyladenosine (m6A) Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
1 的
1 review
Showing All
Filter By:
-
Application: ImmunoprecipitationSample Tested: MDA-MB-231Species: HumanVerified Customer | Posted 07/17/2025RNA immunoprecipitation (RIP) was performed using either IgG or m6A Antibody (NBP3-18690) (2 ug/IP) with 50 ug MDA-MB-231 total RNA, followed by qPCR with primers for PRSS23 gene.
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Loading...