NDUFS7 Overexpression Lysate

Name: NDUFS7 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-13565
Price: 689 USD
Availability: InStock

Novus Biologicals | Catalog # NBL1-13565

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for NDUFS7 Overexpression Lysate

NDUFS7 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC203279

Accession#: NM_024407

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug) and 1 vial of empty vector negative control (100ug). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml).

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate.

Theoretical MW

23.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for NDUFS7 Overexpression Lysate

Western Blot: NDUFS7 Overexpression Lysate [NBL1-13565]

Western Blot: NDUFS7 Overexpression Lysate (Adult Normal) [NBL1-13565] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NDUFS7.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: NDUFS7

This gene encodes a protein that is a subunit of one of the complexes that forms the mitochondrial respiratory chain. This protein is one of over 40 subunits found in complex I, the nicotinamide adenine dinucleotide (NADH):ubiquinone oxidoreductase. This complex functions in the transfer of electrons from NADH to the respiratory chain, and ubiquinone is believed to be the immediate electron acceptor for the enzyme. Mutations in this gene cause Leigh syndrome due to mitochondrial complex I deficiency, a severe neurological disorder that results in bilaterally symmetrical necrotic lesions in subcortical brain regions. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Alternate Names

CI-20, CI-20KD, complex I, mitochondrial respiratory chain, 20-KD subunit, Complex I-20kD, EC 1.6.5.3, EC 1.6.99.3, EC 1.6.99.5, FLJ45860, FLJ46880, MGC120002, MY017, NADH dehydrogenase (ubiquinone) Fe-S protein 7 (20kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Qreductase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial, NADH:ubiquinone oxidoreductase PSST subunit, NADH-coenzyme Q reductase, NADH-ubiquinone oxidoreductase 20 kDa subunit, PSST subunit, PSSTcomplex I 20kDa subunit

Gene Symbol

NDUFS7

Additional NDUFS7 Products

Product Documents for NDUFS7 Overexpression Lysate

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Certificate of Analysis

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Product Specific Notices for NDUFS7 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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