Recombinant Human NECAP2 His Protein

Novus Biologicals | Catalog # NBP1-51043

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
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Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-263 of Human NECAP2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEESGYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA KGQMAYIKLE DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL GFKEGQTIKL NIANMKKKEG AAGNPRVRPA STGGLSLLPP PPGGKTSTLI PPPGEQLAVG GSLVQPAVAP SSGGAPVPWP QPNPATADIW GDFTKSTGST SSQTQPGTGW VQF

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

30.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NECAP2 His Protein

SDS-PAGE: Recombinant Human NECAP2 His Protein [NBP1-51043]

SDS-PAGE: Recombinant Human NECAP2 His Protein [NBP1-51043]

SDS-Page: Recombinant Human NECAP2 Protein [NBP1-51043] - 15 % SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-51043
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2 mM DTT, 0.1 M NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NECAP2

NECAP2, also known as adaptin ear-binding coat-associated protein 2, is a essential protein paralogues for clathrin-mediated membrane trafficking that are enriched in CCV coats. This protein colocalizes with AP-2 at the plasma membrane by binding AP-2s alpha-ear domain, and interacts with AP-1, AP-2 and several GAE domain proteins termed GGA1, GGA2 and GGA3. NECAP2 is Involved in endocytosis. Recombinant human NECAP2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

adaptin ear-binding coat-associated protein 2, adaptin-ear-binding coat-associated protein 2, FLJ10420, NECAP endocytosis associated 2, NECAP endocytosis-associated protein 2, NECAP-2

Gene Symbol

NECAP2

Additional NECAP2 Products

Product Documents for Recombinant Human NECAP2 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human NECAP2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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