NEDD4 Antibody (JE35-58)

Novus Biologicals | Catalog # NBP3-32641

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE35-58 expressed in HEK293
View all NEDD4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment corresponding to Human NEDD4 aa 160-460. (Uniprot: P46934)

Localization

Cytoplasm, Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

149 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NEDD4 Antibody (JE35-58)

NEDD4 Antibody (JE35-58)

Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641]

Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641] - Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (NBP3-32641) at 1/2,000 dilution.

Lane 1: U87MG cell lysate
Lane 2: A549 cell lysate
Lane 3: NIH/3T3 cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 149 kDa
Observed band size: 110 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32641) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
NEDD4 Antibody (JE35-58) Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641]

Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641]

Western blot analysis on different lysates with NBP3-32641 at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 8 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP3-32641, 1/2,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 149 kDa Observed band size: 149 kDa

Applications for NEDD4 Antibody (JE35-58)

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEDD4

Nedd4 is one of a group of mouse genes that show developmentally regulated expression in mouse embryonic brain. Nedd4 is expressed in various other embryonic tissues and persists in most adult tissues.

Long Name

Neural Precursor Cell Expressed, Developmentally Down-regulated Protein 4

Alternate Names

RPF1

Gene Symbol

NEDD4

Additional NEDD4 Products

Product Documents for NEDD4 Antibody (JE35-58)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NEDD4 Antibody (JE35-58)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases
Notch Pathway

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