NEDD4 Antibody

Novus Biologicals | Catalog # NBP2-27197

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Equine, Primate

Predicted:

Monkey (100%), Zebrafish (94%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all NEDD4 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 950-1000 of human NEDD4 was used as the immunogen for this antibody.

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 94% homologous in Zebrafish, and 88% homologous in Chicken and Opossum.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NEDD4 Antibody

Western Blot: NEDD4 Antibody [NBP2-27197]

Western Blot: NEDD4 Antibody [NBP2-27197]

Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for NEDD4 Antibody

Application
Recommended Usage

Western Blot

4-6ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEDD4

The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein. NEDD4 contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains. It is involved in the pathway leading to the degradation of VEGFR-2/kDFR, independently of its ubiquitin-ligase activity. NEDD4 acts as a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. It is also reported to be involved in the budding of many viruses and is the regulator of epithelial Na+ channels, which lead to arterial hypertension. NEDD4 protein is localized to the sarcolemmal region of muscle fibers. Increase in NEDD4 protein expression leads to unloaded and denervated muscles as well as a decrease in Notch1 expression. Hence, NEDD4 acts as down regulator of Notch-1 gene expression.

Long Name

Neural Precursor Cell Expressed, Developmentally Down-regulated Protein 4

Alternate Names

RPF1

Gene Symbol

NEDD4

UniProt

P46934 (Human)

Additional NEDD4 Products

Product Documents for NEDD4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NEDD4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases
Notch Pathway

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