Nicastrin Antibody (019) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89828

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 019 expressed in HEK293

Format

Azide and BSA Free
View all Nicastrin Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Nicastrin (Accession#: NP_056146.1; Met1-Glu669).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Nicastrin Antibody (019) - Azide and BSA Free

Western Blot: Nicastrin Antibody (019) [NBP2-89828]

Western Blot: Nicastrin Antibody (019) [NBP2-89828]

Western Blot: Nicastrin Antibody (019) [NBP2-89828] - Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell LysateLane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:78 kDa Observed band size:170 kDa (We are unsure as to the identity of these extra bands.)
Immunoprecipitation: Nicastrin Antibody (019) [NBP2-89828]

Immunoprecipitation: Nicastrin Antibody (019) [NBP2-89828]

Immunoprecipitation: Nicastrin Antibody (019) [NBP2-89828] - Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-NCSTN rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NCSTN rabbit monoclonal antibody,at 1:150 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 78 kDa Observed band size: 130 kDa

Applications for Nicastrin Antibody (019) - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nicastrin

Nicastrin is a large protein that is a component of an enzyme called gamma-secretase, which is lodged in the cell's membrane. Nicastrin protein is physically associated with presenilin and plays an important role in stabilization and correct localization of presenilin to the membrane-bound gamma-secretase complex. Nicastrin also serves as a docking site for gamma-secretase substrates such as APP and Notch, directly binding to them and presenting them properly to gamma-secretase to ensure the correct cleavage process.

Alternate Names

APH2, NCSTN

Gene Symbol

NCSTN

Additional Nicastrin Products

Product Documents for Nicastrin Antibody (019) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Nicastrin Antibody (019) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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