NMDAR2B Antibody (17) - BSA Free
Novus Biologicals | Catalog # NBP3-43543
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Rat
Applications
X-Ray Crystallography
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 17
Format
BSA Free
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Product Specifications
Immunogen
Monoclonal antibodies that bind rat GluN2B ATD were obtained by immunizing mice with the purified intact GluN1-GluN2B NMDA receptors. IgGs were purified from hybridoma cell culture supernatant by Protein-A Sepharose. Fab fragments have been prepared by papain proteolysis followed by re-chromatographing onto Protein-A Sepharose to remove Fc. (Uniprot# Q00960)
Specificity
Reacts specifically with rat GluN2B ATD.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for NMDAR2B Antibody (17) - BSA Free
Immunocytochemistry/ Immunofluorescence: NMDAR2B Antibody (17) - BSA Free [NBP3-43543] -
Immunocytochemistry/ Immunofluorescence: NMDAR2B Antibody (17) - BSA Free [NBP3-43543] - Immunofluorescence analysis of paraformaldehyde fixed C6 cells stained with NBP3-43543 at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43543, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
Flow Cytometry: NMDAR2B Antibody (17) - BSA Free [NBP3-43543] -
Flow Cytometry: NMDAR2B Antibody (17) - BSA Free [NBP3-43543] - Paraformaldehyde fixed C6 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-43543 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Applications for NMDAR2B Antibody (17) - BSA Free
Application
Recommended Usage
X-Ray Crystallography
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NMDAR2B
Long Name
Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B
Alternate Names
glutamate [NMDA] receptor subunit epsilon-2, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B, hNR3, MGC142180, NMDA R, NR2B Subunit, NMDAR2B, NMDAR2BMGC142178, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3
Gene Symbol
GRIN2B
Additional NMDAR2B Products
Product Documents for NMDAR2B Antibody (17) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NMDAR2B Antibody (17) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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