Nodal Modulator (NOMO, also known as pM5) is a 130 kDa type I membrane protein with no recognizable functional motif. NOMO is a binding partner of Nicalin, a nicastin-related protein. The Nicalin/NOMO complex is localized in the endoplasmic reticulum and functions as an antagonist for Nodal and Activin signaling. Three copies of the NOMO gene, encoding NOMO isoforms 1, 2 and 3 have been described. The extracellular domains (ECD) of the isoforms share 99% amino acid sequence homology. The ECD of human NOMO and mouse NOMO share 94% amino acid sequence homology.
NOMO Antibody [Janelia Fluor® 669]
Novus Biologicals | Catalog # AF3755JF669
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Janelia Fluor 669
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human NOMO
Ser32- Asp1155
Accession # Q15155
Ser32- Asp1155
Accession # Q15155
Specificity
Detects human NOMO in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for NOMO Antibody [Janelia Fluor® 669]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: NOMO
Long Name
NODAL Modulator
Alternate Names
NOMO2, PM5
Gene Symbol
NOMO2
Additional NOMO Products
Product Documents for NOMO Antibody [Janelia Fluor® 669]
Product Specific Notices for NOMO Antibody [Janelia Fluor® 669]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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