NR12S

Key Product Details

Product Description

Key information: NR12S is a fluorogenic lipid membrane dye that stains the outer leaflet of lipid vesicles and cell membranes. Suitable for live cell imaging.

Used for: monitoring cholesterol and lipid order selectively at the outer leaflet of cell membranes.

Application: fluorescence microscopy.

Properties and Photophysical Data: NR12S is a conjugation of the Nile Red fluorophore with a long alkyl chain and a zwitterionic group, allowing strong interactions with the lipid membranes. Excitation and emission maxima (λ) are 554 nm and 627 nm in DMSO, respectively; quantum yield = 0.55.

Optical Data

Product Specifications for NR12S

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for NR12S

The following data is based on the product molecular weight 695.96.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	14.37 mL	71.84 mL	143.69 mL
0.5 mM	2.87 mL	14.37 mL	28.74 mL
1 mM	1.44 mL	7.18 mL	14.37 mL
5 mM	0.29 mL	1.44 mL	2.87 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 8 publications citing the usage of this product.

• Kucherak Switchable nile red-based probe for cholesterol and lipid order at the outer leaflet of biomembranes. J.Am.Chem.Soc. 2010 PMID: 20225874

Product Documents for NR12S