Nse2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-29989

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Nse2 Primary Antibodies »

Product Specifications

Immunogen

Between 1 and 50

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nse2 Antibody - BSA Free

Nse2 Antibody

Immunoprecipitation:Nse2 AntibodyNBP3-29989] -

Immunoprecipitation:Nse2 AntibodyNBP3-29989] -Detection of human MMS21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MMS21 antibody NBP3-29989 (lot NBP3-29989-2) used for IP at 6 µg per reaction. MMS21 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-29989-1) and rabbit anti-MMS21.. For blotting immunoprecipitated MMS21, NBP3-29989 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for Nse2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Nse2

E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks byhomologous recombination. The complex may promote sister chromatid homologous recombination by recruiting theSMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteinssuch as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is requiredfor the prevention of DNA damage-induced apoptosis by facilitating DNA repair

Alternate Names

C8orf36, E3 SUMO-protein ligase NSE2, EC 6.3.2, EC 6.3.2.-, FLJ32440, hMMS21, methyl methanesulfonate sensitivity gene 21, MMS21 homolog, MMS21chromosome 8 open reading frame 36, Non-SMC element 2 homolog, non-SMC element 2 homolog (MMS21, S. cerevisiae), non-SMC element 2, MMS21 homolog (S. cerevisiae), Non-structural maintenance of chromosomes element 2 homolog, NSE2

Gene Symbol

NSMCE2

Additional Nse2 Products

Product Documents for Nse2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Nse2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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