NSF Antibody - Azide Free

Novus Biologicals | Catalog # NB300-733

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Rat

Predicted:

Human (100%), Mouse (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all NSF Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # Q9QUL6

Reactivity Notes

It is anticipated that the antibody will also work with human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Localization

Cytoplasmic

Specificity

Specific for endogenous levels of the ~75 kDa NSF protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NSF Antibody - Azide Free

Western Blot: NSF Antibody [NB300-733]

Western Blot: NSF Antibody [NB300-733]

Western Blot: NSF Antibody [NB300-733]

Applications for NSF Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NSF

NSF is a critical component of the SNARE (soluble NSF attachment protein receptors) protein complex that is involved in synaptic vesicle trafficking. Specifically, NSF has been found to be essential in membrane fusion. Furthermore, NSF has been recently demonstrated to bind other protein complexes such as AMPA receptor subunits (GluR2), GATE-16, LMA-1 and Rabs suggesting a more diverse role in the assembly of various protein complexes (Whiteheart et al., 2004).

Long Name

N-ethylmaleimide sensitive factor, vesicle fusing ATPase

Alternate Names

DEE96, SEC18, SKD2

Entrez Gene IDs

4905 (Human); 18195 (Mouse); 60355 (Rat)

Gene Symbol

NSF

UniProt

Q9QUL6 (Rat)

Additional NSF Products

Product Documents for NSF Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for NSF Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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