Nuclear Extraction Kit

Novus Biologicals | Catalog # NBP2-29447

Novus Biologicals
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Key Product Details

Applications

Western Blot

Kit Type

Extraction Kit

Product Summary for Nuclear Extraction Kit

The Nuclear Extraction Kit provides a simple and convenient method for the isolation of nuclear and cytoplasmic extracts from mammalian cells and tissue samples. This procedure is relevant to the monitoring of translocation of cell signaling molecules from cytoplasm to the nucleus. Examples include translocation of NF-kB molecules to the nucleus in TNF-alpha treated cells, and translocation of mitogen-activated protein kinase to the nucleus in growth factor treated cells.

The Nuclear Extraction Kit can be used in the preparation of purified proteins for use in Western blotting, Eletrophoretic Mobility Assays (EMSA) and preparative purification of nuclear proteins.

Scientific Data Images for Nuclear Extraction Kit

Western Blot: Nuclear Extraction Kit [NBP2-29447]

Western Blot: Nuclear Extraction Kit [NBP2-29447]

Western Blot: Nuclear Extraction Kit [NBP2-29447] - 20 ug of HeLa cell proteins separated on 4-20% SDS-PAGE. Lane 1: Nuclear extract. Lane 2: Cytoplasmic extract. Lane 3: Total cell lysate.

Kit Contents for Nuclear Extraction Kit

  • 10% Detergent Solution (10 mL)
  • 100 mM PMSF (10 mL)
  • 100X Protease Inhibitor Cocktail - PIC (100 uL)
  • 10X Hypotonic Lysis Buffer (10 mL)
  • 10X PBS (2 x 50 mL)
  • 1M DTT for nuclear extraction from tissue (100 uL)
  • 1X Nuclear Extraction Buffer (10 mL)

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Storage of components varies. See protocol for specific instructions.

Background: Nuclear

Nuclear Extraction

Alternate Names

Nuclear Extraction, Nuclear Extraction Kit

Additional Nuclear Products

Product Documents for Nuclear Extraction Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Nuclear Extraction Kit

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Citations for Nuclear Extraction Kit

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Protocols

View specific protocols for Nuclear Extraction Kit (NBP2-29447):


Material Safety Data Sheet for PMSF

Hazard Information
Chemical Name: Phenylmethylsulfonyl fluoride
Chemical Formula: C7H7FO2S
CAS Number: 329-98-6

First Aid Measures
Eye Contact: Can cause slight eye irritation.
Skin Contact: Can cause slight skin irritation.
Inhalation: Can cause slight respiratory tract irritation.
Ingestion: Harmful if swallowed.

Accidental Release Measures
If inhaled, move person into fresh air. If not breathing give artificial respiration and consult a physician.
In case of skin contact, wash off with soap and plenty of water.
In case of eye contact, rinse thoroughly with plenty of water for at least 15 minutes.
If swallowed do NOT induce vomiting. Rinse mouth with water and consult a physician.
Handling and Storage
Handling: Avoid contact with skin and eyes. Keep container tightly closed in a cool, dry, well-ventilated place.
Exposure Controls / Personal Protection
Ventilation: Handle in a well-ventilated area
Gloves: Handle with rubber or latex gloves
Eye Protection: Safety glasses, goggles or face shield

Physical and Chemical Properties
Form: Solid
Color: No data available
Odor: No data available
Melting Point: 92 degrees C (197.6 degrees F)
Boiling Temperature: No data available
Density: No data available
Vapor Pressure: No data available
Solubility in Water: soluble
Flash Point: No data available
Explosion limits: No data available
Ignition Temperature: No data available

Stability and Reactivity
Stable under recommended storage conditions.

Disposal Considerations
Absorb spill and place in a container for disposal according to local regulations.

Material Safety Data Sheet for Protease Inhibitor Cocktail

Hazard Information
Chemical Name: 4-(2-Aminoethyl) benzenesulfonylfluoride hydrochloride
Chemical Formula: C8H10FNO2S HCl
CAS Number: 30827-99-7

First Aid Measures
Eye Contact: Can cause slight eye irritation.
Skin Contact: Can cause slight skin irritation.
Inhalation: Can cause slight respiratory tract irritation.
Ingestion: Harmful if swallowed.

Accidental Release Measures
If inhaled, move person into fresh air. If not breathing give artificial respiration and consult a physician.
In case of skin contact, wash off with soap and plenty of water.
In case of eye contact, rinse thoroughly with plenty of water for at least 15 minutes.
If swallowed do NOT induce vomiting. Rinse mouth with water and consult a physician.

Handling and Storage
Handling: Avoid contact with skin and eyes. Keep container tightly closed in a cool, dry, well-ventilated place.

Exposure Controls / Personal Protection
Ventilation: Handle in a well-ventilated area
Gloves: Handle with rubber or latex gloves
Eye Protection: Safety glasses, goggles or face shield

Physical and Chemical Properties
Form: Solid
Color: beige
Odor: No data available
Melting Point: 183 degrees C (361 degrees F)
Boiling Temperature: No data available
Density: No data available
Vapor Pressure: No data available
Solubility in Water: soluble
Flash Point: No data available
Explosion limits: No data available
Ignition Temperature: No data available

Stability and Reactivity
Stable under recommended storage conditions.

Disposal Considerations
Absorb spill and place in a container for disposal according to local regulations.

Material Safety Data Sheet for Igepal CA-630 (NP-40)

Hazard Information
Chemical Name: Igepal CA-630
Chemical Formula: a-[(1,1,3,3-Tetramethylbutyl)phenyl]-w-hydroxy-poly(oxy-1,2-ethanediyl)
CAS Number: 9036-19-5

First Aid Measures
Eye Contact: Can causes eye irritation.
Skin Contact: Causes skin irritation and is toxic if absorbed through skin.
Inhalation: May be harmful if inhaled. Causes respiratory tract irritation.
Ingestion: Harmful if swallowed.

Accidental Release Measures
If inhaled, move person into fresh air. If not breathing give artificial respiration and consult a physician.
In case of skin contact, wash off with soap and plenty of water.
In case of eye contact, rinse thoroughly with plenty of water for at least 15 minutes.
If swallowed do NOT induce vomiting. Rinse mouth with water and consult a physician.

Handling and Storage
Handling: Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Keep container tightly closed in a cool, dry, well-ventilated place.

Exposure Controls / Personal Protection
Ventilation: Handle in a fume hood to avoid vapors
Gloves: Handle with rubber or latex/nitrile gloves
Eye Protection: Safety goggles

Physical and Chemical Properties
Form: Liquid
Color: No data available
Odor: No data available
Melting Point: No data available
Boiling Temperature: No data available
Density: 1.06 g/mL at 25 degrees C (77 degrees F)
Vapor Pressure: No data available
Solubility in Water: Soluble
Flash Point: No data available
Explosion limits: No data available
Ignition Temperature: No data available

Stability and Reactivity
Stable under recommended storage conditions.

Disposal Considerations
Absorb spill and place in a container for disposal according to local regulations.

Material Safety Data Sheet for Dithiothreitol (DTT)
Hazard Information
Chemical Name: Dithiothreitol
Chemical Formula: C4H10O2S2
CAS Number: 3483-12-3

First Aid Measures
Eye Contact: Can causes eye irritation.
Skin Contact: Causes skin irritation and is toxic if absorbed through skin.
Inhalation: May be harmful if inhaled. Causes respiratory tract irritation.
Ingestion: Harmful if swallowed.

Accidental Release Measures
If inhaled, move person into fresh air. If not breathing give artificial respiration and consult a physician.
In case of skin contact, wash off with soap and plenty of water.
In case of eye contact, rinse thoroughly with plenty of water for at least 15 minutes.
If swallowed do NOT induce vomiting. Rinse mouth with water and consult a physician.

Handling and Storage
Handling: Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Keep container tightly closed in a cool, dry, well-ventilated place.
Exposure Controls / Personal Protection
Ventilation: Handle in a fume hood to avoid vapors
Gloves: Handle with rubber or latex/nitrile gloves
Eye Protection: Safety goggles
Physical and Chemical Properties
Form: Liquid
Color: No data available
Odor: No data available
Melting Point: No data available
Boiling Temperature: No data available
Density: No data available
Vapor Pressure: No data available
Solubility in Water: Soluble
Flash Point: NA
Explosion limits: NA
Ignition Temperature: No data available

Stability and Reactivity
Stable under recommended storage conditions.

Disposal Considerations
Absorb spill and place in a container for disposal according to local regulations.

Material Safety Data Sheet for TRITON X-100

Hazard Information
Chemical Name: TRITON X-100
Chemical Formula: (C2-H4-O)nC14-H22-O
CAS Number: 9002-93-1
EEC-No: n/a

First Aid Measures
Eye Contact: Can causes eye irritation.
Skin Contact: Causes skin irritation and is toxic if absorbed through skin.
Inhalation: May be harmful if inhaled. Causes respiratory tract irritation.
Ingestion: Harmful if swallowed.

Accidental Release Measures
If inhaled, move person into fresh air. If not breathing give artificial respiration and consult a physician.
In case of skin contact, wash off with soap and plenty of water.
In case of eye contact, rinse thoroughly with plenty of water for at least 15 minutes.
If swallowed do NOT induce vomiting. Rinse mouth with water and consult a physician.

Handling and Storage
Handling: Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Handle powder in a fume hood. Keep container tightly closed in a cool, dry, well-ventilated place.

Exposure Controls / Personal Protection
Ventilation: Handle in a well-ventilated area
Gloves: Handle with rubber or latex gloves
Eye Protection: Safety glasses, goggles or face shield to protect from splash hazard

Physical and Chemical Properties
Form: Liquid
Color: Colorless
Odor: Odorless
Melting Point: 6 degrees C (42.8 degrees F)
Boiling Temperature: 270 degrees C (518 degrees F)
Density: No data available
Vapor Pressure: No data available
Solubility in Water: Very soluble
Flash Point: No data available
Explosion limits: No data available
Ignition Temperature: No data available

Stability and Reactivity
Stable under recommended storage conditions.
Disposal/Spill Considerations
Absorb spill and place in a container for disposal according to local regulations.

Material Safety Data Sheet for EDTA

Hazard Information
Chemical Name: Ethylenediaminetetraacetic Acid Tetrasodium Salt, Dihydrate
Chemical Formula: C10H12N2Na4O8.2H2O
CAS Number: 10378-23-1

First Aid Measures
Eye Contact: Can cause slight eye irritation.
Skin Contact: Can cause slight skin irritation.
Inhalation: Can cause slight respiratory tract irritation.
Ingestion: Harmful if swallowed.

Accidental Release Measures
If inhaled, move person into fresh air. If not breathing give artificial respiration and consult a physician.
In case of skin contact, wash off with soap and plenty of water.
In case of eye contact, rinse thoroughly with plenty of water for at least 15 minutes.
If swallowed do NOT induce vomiting. Rinse mouth with water and consult a physician.
Handling and Storage
Handling: Avoid contact with skin and eyes. Keep container tightly closed in a cool, dry, well-ventilated place.

Exposure Controls / Personal Protection
Ventilation: Handle in a well-ventilated area
Gloves: Handle with rubber or latex gloves
Eye Protection: Safety glasses, goggles or face shield

Physical and Chemical Properties
Form: Solid
Color: White
Odor: No data available
Melting Point: No data available
Boiling Temperature: No data available
Density: No data available
Vapor Pressure: No data available
Solubility in Water: soluble
Flash Point: > 93.3 degrees C (200 degrees F)
Explosion limits: No data available
Ignition Temperature: No data available

Stability and Reactivity
Stable under recommended storage conditions.

Disposal Considerations
Absorb spill and place in a container for disposal according to local regulations.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Nuclear Extraction Kit

Showing  1 - 1 的 1 FAQ Showing All

  • Q: We are looking to isolate mtDNA that is free from nuclear DNA and nuclear DNA that is free from mtDNA. Do you know if these kits (NBP2-29447, NBP2-29448) will be able to do this? We are wanting the isolated DNA to use as a positive control for a copy number experiment.

    A: You would need to use both kits in order to seperate mtDNA and nDNA. I was worried about the lysis buffer contained in both kits would force the DNA to precipitate, this can occur if the buffer is very basic or alcohol based. I also double checked to make sure all the buffers contained within the kit do not have any DNAses or RNAses present. Follow the protocol for both kits until you use the mitochondrial/nuclear lysis buffer and then proceed to collect the individual supernatants. I would proceed to use our Quik ChIP Kit. The way this kit works is by precipitating the DNA and then running it through a spin column included into the kit. Then the DNA is eluded of the column and the resulting product should be purified mDNA or nDNA depending on which supernatant you used. I reccomend on using a lot of starting material for your nuclear extract as the yield tends to be low, which is typical. I also reccomend to use 18 mega ohm DI water when starting your nuclear/mitochondrial extractions. The nuclear and mitochondrial extraction kits were designed for the purpose of extracting proteins and have not been tested for this particular application.

Showing  1 - 1 的 1 FAQ Showing All