OBFC2A Overexpression Lysate

Name: OBFC2A Overexpression Lysate
Brand: Novus Biologicals
SKU: NBP2-08591
Price: 689 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-08591

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for OBFC2A Overexpression Lysate

OBFC2A Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC203672

Accession#: NM_001031716

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

Theoretical MW

22.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for OBFC2A Overexpression Lysate

Western Blot: OBFC2A Overexpression Lysate [NBP2-08591]

Western Blot: OBFC2A Overexpression Lysate (Adult Normal) [NBP2-08591] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OBFC2A.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: OBFC2A

OBFC2A is also known as hSSB2 or SSB2 and is a homolog of hSSB1 (OBFC2B). OBFC2A, as well as OBFC2B are single-stranded binding proteins that contain an oligonucleotide/oliosaccharide binding fold (OB-fold). OB-fold proteins associate with each other to form multi-OB-fold complexes that associate with DNA-processing complexes that participate in DNA damage repair and replication. OBFC2A has been shown to protect genomic stability, and it is hypothesized that its participation in the DNA damage response may involve RAD51 and the ATM signaling pathway.

Alternate Names

DKFZp667M1322, FLJ13624, FLJ22833, hSSB2, MGC111163, oligonucleotide/oligosaccharide-binding fold containing 2A, Oligonucleotide/oligosaccharide-binding fold-containing protein 2A, Sensor of single-strand DNA complex subunit B2, Sensor of ssDNA subunit B2, SOSS complex subunit B2, SOSS-B2, SSB2Single-stranded DNA-binding protein 2

Gene Symbol

NABP1

Additional OBFC2A Products

Product Documents for OBFC2A Overexpression Lysate

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Certificate of Analysis

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Product Specific Notices for OBFC2A Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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