OG 488, SE
Tocris Bioscience | Catalog # 6257
Key Product Details
Description
Wavelength
Product Description
OG 488, SE is a green fluorescent dye. pH-insensitive in physiological pH range (pKa = 4.6). Can be used to monitor pH change in acidic organelles or pathways. Excitation/emission λ 501/526 nm.Optical Data
| Emission Color | Green |
| Brightness | |
| λabs | 496 nm |
| λem | 516 nm |
| Extinction Coefficient (ε) | 76,000 M-1cm-1 |
| Quantum Yield (φ) | 0.9 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| pH Sensitivity | pH-insensitive in physiological pH range (pKa = 4.6) |
| Correction Factor 280 | 0.12 |
| Application | Imaging, acidic organelle probe, flow, STED |
| Alternative For | TFAX 488, SE, 5(6)-SFX (Fluorescein), SE, FITC, |
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Product Specifications for OG 488, SE
Molecular Weight
Formula
Storage
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 0.51 | 1 |
Preparing Stock Solutions for OG 488, SE
The following data is based on the product molecular weight 509.37.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.01 mM | 196.32 mL | 981.60 mL | 1,963.21 mL |
| 0.05 mM | 39.26 mL | 196.32 mL | 392.64 mL |
| 0.1 mM | 19.63 mL | 98.16 mL | 196.32 mL |
| 0.5 mM | 3.93 mL | 19.63 mL | 39.26 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Vergene Phagosomal pH determination by dual fluorescence flow cytometry. Anal.Biochem. 1998 PMID: 9448851
Product Documents for OG 488, SE
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for OG 488, SE
For research use only
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