P 22077

Tocris Bioscience | Catalog # 4485

USP7 inhibitor
Tocris Bioscience

Key Product Details

Description

USP7 inhibitor
View all Deubiquitinating Enzyme Inhibitors »

Product Description

P 22077 is an inhibitor of ubiquitin-specific protease (USP) 7 (EC50 = 8.6 μM); also inhibits the closely related deubiquitinase (DUB) USP47. Demonstrates downstream inhibition of HDM2 and claspin in vitro. Inhibits USP7-mediated p53 deubiquitination. Blocks deubiquitination of Tip60 (KAT5) histone lysine acetyltransferase.

Product Specifications for P 22077

Molecular Weight

315.32

Formula

C12H7F2NO3S2

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

1-[5-[(2,4-Difluorophenyl)thio]-4-nitro-2-thienyl]-ethanone

CAS Number

1247819-59-5

PubChem ID

46931953

InChI Key

RMAMGGNACJHXHO-UHFFFAOYSA-N

SMILES

CC(C1=CC([N+]([O-])=O)=C(SC2=CC=C(F)C=C2F)S1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 31.53 100

Preparing Stock Solutions for P 22077

The following data is based on the product molecular weight 315.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.17 mL 15.86 mL 31.71 mL
5 mM 0.63 mL 3.17 mL 6.34 mL
10 mM 0.32 mL 1.59 mL 3.17 mL
50 mM 0.06 mL 0.32 mL 0.63 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 8 publications citing the usage of this product.

Product Documents for P 22077

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for P 22077

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Customer Reviews for P 22077 (2)

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  • P22077 was seen to induce tumor cell death
    Name: Anonymous
    Species: Human
    Verified Customer | Posted 12/14/2019
    P22077 was seen to induce (tumor) cell death with EC50 values in the low micromolar range. P22077 inhibition exhibited changes in ubiquitylated protein levels that are distinct from the broad specificity inhibitor.
    P 22077 4485
  • USP7 inhibitors 2 (P22077)
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 12/10/2019
    HEK293T cells stably expressing the STF-Luc reporter were pretreated with increasing amounts of USP7 inhibitors P22077
    P 22077 4485

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