pCLNDX Retrovirus Expression Vector

The RetroMax system is designed for maximal virus titer in 293 cells. It takes advantages of two properties of 293 cells, i) high level of transfectibility, ii) strong E1A-mediated stimulation of CMV promoter controlled transcription. 293 cells are of nonmurine origin, hence the problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells) are avoided. Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells to permit infection of more than 50% of dividing target cells in culture.
For amplification, transform DH-5alpha or similar bacteria and plate on LB plates containing 50 ug/ml ampicillin.

10 ug in 20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at -20C. Avoid freeze-thaw cycles.