pCLXSN Retrovirus Expression Vector

Name: pCLXSN Retrovirus Expression Vector
Brand: Novus Biologicals
SKU: NBP2-29500
Price: 269 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-29500

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Product Documents

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Product Specifications

Application Notes

The RetroMax system is designed for maximal virus titer in 293 cells. It takes advantages of two properties of 293 cells, i) high level of transfectibility, ii) strong E1A-mediated stimulation of CMV promoter controlled transcription. 293 cells are of nonmurine origin, hence the problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells) are avoided. Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells to permit infection of more than 50% of dividing target cells in culture.
For amplification, transform DH-5a or similar bacteria and plate on LB plates containing 50 mg/ml ampicillin.

Scientific Data Images for pCLXSN Retrovirus Expression Vector

pCLXSN Retrovirus Expression Vector [NBP2-29500] - Schematic presentation of pCLXSN vector: CMV immediate early enhancer-promoter allows highefficiency transcription in 293 cells. However, this is lost during viral replication. Hence, the gene of interest cloned into Eco RI, Xho I, or Bam HI cloning site is driven by Moloney MLV and murine sarcoma virus LTR (RU5).

Formulation, Preparation, and Storage

Formulation

10 ug in 20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: pCLXSN Retrovirus Expression Vector

The pCLXSN expression vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. All members of the RetroMax expression vector family (pCLXSN, pCLNCX, pCLNRX, and pCLNDX) have an extended packaging signal (omega+) and are derived from safetymodified retrovirus vectors in which the gag open reading frame has been stopped by a point mutation (1), thereby minimizing the opportunity for replication competent retrovirus production by recombination with packaging genome.

Alternate Names

pCLXSN, pCLXSN Retrovirus, Retrovirus, Retrovirus Expression Vector

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Product Specific Notices for pCLXSN Retrovirus Expression Vector

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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