PCTAIRE1 Antibody (JE64-55)
Novus Biologicals | Catalog # NBP3-32727
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE64-55 expressed in HEK293
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Product Specifications
Immunogen
Recombinant protein within Human PCTAIRE1 aa 1-150 / 496. (Uniprot: Q00536)
Localization
Cytoplasm, Cytoplasmic vesicle, secretory vesicle, Cell membrane, Synapse, synaptosome.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PCTAIRE1 Antibody (JE64-55)
Western Blot: PCTAIRE1 Antibody (JE64-55) [NBP3-32727]
Western blot analysis on different lysates with NBP3-32727 at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: U-87 MG cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lane 7: Rat pancreas tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 56/63 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32727 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.Applications for PCTAIRE1 Antibody (JE64-55)
Application
Recommended Usage
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PCTAIRE1
Long Name
Cyclin-dependent kinase 16
Alternate Names
CDK16, PCTK1
Gene Symbol
CDK16
Additional PCTAIRE1 Products
Product Documents for PCTAIRE1 Antibody (JE64-55)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PCTAIRE1 Antibody (JE64-55)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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