PEI STAR-Go™ transfection reagent

Key Product Details

Product Description

PEI STAR-Go™ is a ready-to-use aqueous formulation of our high performance PEI STAR™ transfection reagent (Cat. No. 7854), provided as a sterile-filtered solution. PEI STAR™ is a chemically-defined, linear polyethylenimine (PEI) transfection reagent for affordable and scalable transient gene expression. PEI is a synthetic polymer with an exceptionally high positive charge density in pH-neutral solutions. Positively charged PEI binds strongly to negatively charged DNA and imparts a net cationic charge, allowing the DNA to enter cells. PEI is a non-viral vector commonly used to transfect HEK293, CHO, and a range of other eukaryotic cell lines. Applications include production of lentivirus and rAAV, recombinant proteins, and antibodies. Supplied as a 1mg/mL aqueous solution.

For more information on how to use PEI STAR-Go™ transfection reagent, see our protocols: Gene Expression in Adherent HEK293 Cells, Gene Expression in CHO Suspensions, rAAV Production in Adherent HEK293 Cells, Gene Expression in HEK293 Suspensions, Lentivirus Production with PEI STAR™, rAAV Production in Suspension HEK293 Cells.

PEI STAR-Go is a trademark of Bio-Techne Corp.

Product Specifications for PEI STAR-Go™ transfection reagent

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Calculators

Background References

References are publications that support the biological activity of the product.

• Han Aberrant role of pyruvate kinase M2 in the regulation of gamma-secretase and memory deficits in Alzheimer's disease. Cell Rep. 2021 PMID: 34879266
• Trivedi Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Mol.Ther.Methods Clin.Dev. 2021 PMID: 35071688
• Longo Transient mammalian cell transfection with polyethylenimine (PEI). Methods Enzymol. 2013 PMID: 24011049
• Huang AAV2 production with optimized N/P ratio and PEI-mediated transfection results in low toxicity and high titer for in vitro and in vivo applications. J.Virol.Methods 2013 PMID: 23791963
• Boussif A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc.Natl.Acad.Sci.U.S.A. 1995 PMID: 7638184

Product Documents for PEI STAR-Go™ transfection reagent