PFI 3

Key Product Details

Product Description

PFI 3 is a potent and selective polybromo 1 (PBRM1) and SMARCA4 inhibitor (K_d values are 48 and 89 nM respectively) that also inhibits SMARCA2. This compoud displays 30-fold selectivity over other sub-family branches. PFI 3 accelerates FRAP recovery in cells at a concentration of 1 μM.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the PFI 3 probe summary on the SGC website.

External Portal Information

Chemicalprobes.org

is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of PFI 3 is reviewed on the chemical probes website.

Product Specifications for PFI 3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for PFI 3

The following data is based on the product molecular weight 321.37.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

3 2 1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.11 mL	15.56 mL	31.12 mL
5 mM	0.62 mL	3.11 mL	6.22 mL
10 mM	0.31 mL	1.56 mL	3.11 mL
50 mM	0.06 mL	0.31 mL	0.62 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 8 publications citing the usage of this product.

• Gerstenberger Identification of a chemical probe for family VIII bromodomains through optimization of a fragment hit. J.Med.Chem. 2016 PMID: 27115555
• Vangamudi The SMARCA2/4 ATPase domain surpasses the bromodomain as a drug target in SWI/SNF mutant cancers: Insights from cDNA rescue and PFI-3 inhibitor studies Cancer Res. 2015 PMID: 26139243

Product Documents for PFI 3