PFI 7

Key Product Details

Product Description

PFI 7 is a selective GID4 antagonist (K_d = 80 nM by SPR assays and EC₅₀ = 0.6 μM by cell-based NanoBRET). PFI 7 binds within the beta -barrel of GID4 substrate binding pocket, disrupting its interaction with the canonical Pro/N-degron peptide in live cells. It alters protein levels of several proteins including RNA helicases as measured by quantitative proteomics.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the PFI 7 probe summary on the SGC website.

Product Specifications for PFI 7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for PFI 7

The following data is based on the product molecular weight 474.43.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.11 mL	10.54 mL	21.08 mL
5 mM	0.42 mL	2.11 mL	4.22 mL
10 mM	0.21 mL	1.05 mL	2.11 mL
50 mM	0.04 mL	0.21 mL	0.42 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Owens A chemical probe to modulate human GID4 Pro/N-degron interactions. Nat.Chem.Biol. 2024 PMID: 38773330

Product Documents for PFI 7