phospho-GAP-43 (S41) Antibody

R&D Systems | Catalog # PPS006

R&D Systems
Discontinued Product
PPS006 has been discontinued. View all GAP-43 products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Xenopus, Zebrafish

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GAP-43 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S41 of GAP-43

Specificity

Specific for the ~50 kDa GAP-43 protein phosphorylated at S41 in Western blots. In some tissues the antibody also recognizes a higher molecular weight protein, that is also recognized by the Pan GAP-43 antibody. This may be a GAP-43 aggregate or oligomer.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for phospho-GAP-43 (S41) Antibody

Detection of Phospho-GAP-43 (S41) antibody by Western Blot.

Detection of Phospho-GAP‑43 (S41) by Western blot.

Western blot of rat cortex lysate showing specific immunolabeling of the ~50 kDa GAP-43 protein phosphorylated at S41 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-GAP-43 (S41). The immunolabeling is completely eliminated by treatment with lambda -PPase.
Detection of Mouse GAP-43 by Western Blot

Detection of Mouse GAP-43 by Western Blot

GAP43 expression is downregulated in CGRP-positive neurons upon loss of adipose mTORC2. (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n = 6; 6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n = 6; 6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n = 5; 5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 (GAP43)-pS41 and calcitonin gene-related peptide (CGRP). (N = 11; 9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N = 19; 11). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36028121), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse GAP-43 by Western Blot

Detection of Mouse GAP-43 by Western Blot

GAP43 expression is downregulated in CGRP-positive neurons upon loss of adipose mTORC2. (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n = 6; 6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n = 6; 6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n = 5; 5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 (GAP43)-pS41 and calcitonin gene-related peptide (CGRP). (N = 11; 9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N = 19; 11). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36028121), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse GAP-43 by Western Blot

Detection of Mouse GAP-43 by Western Blot

GAP43 expression is downregulated in CGRP-positive neurons upon loss of adipose mTORC2. (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n = 6; 6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n = 6; 6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n = 5; 5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 (GAP43)-pS41 and calcitonin gene-related peptide (CGRP). (N = 11; 9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N = 19; 11). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36028121), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Phospho-GAP-43 (S41) by Western Blot

Detection of Mouse Phospho-GAP-43 (S41) by Western Blot

GAP43 expression is downregulated in CGRP-positive neurons upon loss of adipose mTORC2. (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n = 6; 6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n = 6; 6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n = 5; 5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 (GAP43)-pS41 and calcitonin gene-related peptide (CGRP). (N = 11; 9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N = 19; 11). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36028121), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Phospho-GAP-43 (S41) by Western Blot

Detection of Mouse Phospho-GAP-43 (S41) by Western Blot

GAP43 expression is downregulated in CGRP-positive neurons upon loss of adipose mTORC2. (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n = 6; 6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n = 6; 6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n = 5; 5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 (GAP43)-pS41 and calcitonin gene-related peptide (CGRP). (N = 11; 9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N = 19; 11). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36028121), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Phospho-GAP-43 (S41) by Western Blot

Detection of Mouse Phospho-GAP-43 (S41) by Western Blot

GAP43 expression is downregulated in CGRP-positive neurons upon loss of adipose mTORC2. (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n = 6; 6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n = 6; 6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n = 5; 5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 (GAP43)-pS41 and calcitonin gene-related peptide (CGRP). (N = 11; 9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N = 19; 11). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36028121), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Phospho-GAP-43 (S41) by Western Blot

Detection of Mouse Phospho-GAP-43 (S41) by Western Blot

GAP43 expression is downregulated in CGRP-positive neurons upon loss of adipose mTORC2. (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n = 6; 6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n = 6; 6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n = 5; 5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 (GAP43)-pS41 and calcitonin gene-related peptide (CGRP). (N = 11; 9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N = 19; 11). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36028121), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Phospho-GAP-43 (S41) by Western Blot

Detection of Mouse Phospho-GAP-43 (S41) by Western Blot

GAP43 expression is downregulated in CGRP-positive neurons upon loss of adipose mTORC2. (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n = 6; 6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n = 6; 6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n = 5; 5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 (GAP43)-pS41 and calcitonin gene-related peptide (CGRP). (N = 11; 9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N = 19; 11). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36028121), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Phospho-GAP-43 (S41) by Western Blot

Detection of Mouse Phospho-GAP-43 (S41) by Western Blot

GAP43 expression is downregulated in CGRP-positive neurons upon loss of adipose mTORC2. (A) Immunoblot analysis of inguinal WAT (iWAT) tissue from control and iAdRiKO mice two weeks after tamoxifen treatment. (n = 6; 6). (B) Immunoblot analysis of iWAT tissue from control and iAdRiKO mice four weeks after tamoxifen treatment. (n = 6; 6). (C) Immunoblot analysis of surgically denervated iWAT depot (denervation) compared to iWAT depot from sham-operated mice (sham). Neurofilament heavy polypeptide (NFH). (n = 5; 5). (D) Representative image of a large nerve bundle in iWAT of control mice immunostained with growth-associated protein 43 (GAP43)-pS41 and calcitonin gene-related peptide (CGRP). (N = 11; 9). (E) Representative image of a large nerve bundle in iWAT of control mice immunostained with GAP43-pS41 and tyrosine hydroxylase (TH). (N = 19; 11). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36028121), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for phospho-GAP-43 (S41) Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat cortex tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GAP-43

GAP-43 (Growth Associated Protein 43 kDa), also known as Neuromodulin, is a 24 kDa, neuron growth cone-associated member of the neuromodulin family. Apparently due to its tertiary structure, it runs anomalously at 43 kDa on SDS-polyacrylamide gel. Rat GAP-43 is a 226 amino acid (aa) protein that contains an N-terminal lipid binding motif, an 11 aa IQ domain (IQ:Ile-Gln-X-Ser-etc) (aa 38 - 48), and a C-terminus with four potential serine phosphorylation sites. The molecule may utilize the IQ domain to bind calmodulin. Protein kinase C-mediated phosphorylation of the Serine at 41 within this site inhibits this interaction. Unphosphorylated, calmodulin-bound GAP-43 is always associated with neurite retraction, while S41 phosphorylated GAP-43 stabilizes actin filaments and promotes growth cone extension.

Long Name

Growth Associated Protein 43

Alternate Names

B-50, Basp2, GAP43, Neuromodulin

Entrez Gene IDs

2596 (Human); 14432 (Mouse); 29423 (Rat)

Gene Symbol

GAP43

Additional GAP-43 Products

Product Documents for phospho-GAP-43 (S41) Antibody

Certificate of Analysis

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Product Specific Notices for phospho-GAP-43 (S41) Antibody

For research use only

Citations for phospho-GAP-43 (S41) Antibody

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