phospho-MARCKS (S152/S156) Antibody

R&D Systems | Catalog # PPS010

R&D Systems
Discontinued Product
PPS010 has been discontinued. View all MARCKS products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Chicken, Xenopus, Zebrafish

Cited:

Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all MARCKS Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S152/S156 of MARCKS

Specificity

Specific for the ~83 kDa MARCKS protein phosphorylated at S152/S156 in Western blots of rat brain homogenates.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for phospho-MARCKS (S152/S156) Antibody

Detection of Phospho-MARCKS (S152/S156) antibody by Western Blot.

Detection of Phospho-MARCKS (S152/S156) by Western Blot.

Western blot of rat brain lysate showing specific immunolabeling of the ~83 kDa MARCKS protein phosphorylated at S152/S156 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-MARCKS (S152/S156). The immunolabeling is completely eliminated by treatment with lambda -PPase.
Detection of Phospho-MARCKS (S152/S156) by Western Blot

Detection of Phospho-MARCKS (S152/S156) by Western Blot

Decreased protein kinase C (PKC) isoforms in crSTIM1−/− hearts. Immunoblots and densitometric analysis of PKC alpha (a) and MARCKS (b) phosphorylation in hearts isolated from control and crSTIM1−/− mice treated subcutaneously with either 15 min saline or phenylephrine. Protein expression is normalized to total protein and/or GAPDH. STIM1, stromal interaction molecule 1. *p < 0.05, **p < 0.01, versus age‐matched control (ANOVA/Tukey's post hoc), n = 3 per genotype. STIM1, stromal interaction molecule 1 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35179826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for phospho-MARCKS (S152/S156) Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat brain lysate

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: MARCKS

Rat MARCKS (Myristoylated, Alanine-rich C Kinase protein Substrate) is an 83 kDa, 309 amino acid intracellular protein that belongs to the MARCKS family of molecules. It is an actin-organizing molecule and a key substrate for activated protein kinase C (PKC) and PKC-related kinase. MARCKS is normally sequestered on the inner face of the plasma membrane. Here, it is bound to phosphatidylserine (PS) via electrostatic and hydrophobic interactions. Upon integrin ligation ( alpha 5 beta 1), PKC is activated, and phosphorylates MARCKS at S152 and S156. This breaks the PS interaction, forcing MARCKS into the cytosol. Subsequent cytosolic dephosphorylation of MARCKS induces MARCKS-mediated polymerization of actin and its return to the membrane.

Long Name

Myristoylated Alanine-rich Protein Kinase C Substrate

Alternate Names

80K-L, MACS, PRKCSL

Entrez Gene IDs

4082 (Human); 17118 (Mouse); 25603 (Rat)

Gene Symbol

MARCKS

Additional MARCKS Products

Product Documents for phospho-MARCKS (S152/S156) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for phospho-MARCKS (S152/S156) Antibody

For research use only

Related Research Areas

Intracellular Kinases

Citations for phospho-MARCKS (S152/S156) Antibody

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