Phospholamban [p Ser16] Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-18744

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Phospholamban Primary Antibodies »

Product Specifications

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 14-25 of human cardiac phospholamban (RA[pS]TIEMPQQAR-C) with an added C-terminal cysteine

Modification

p Ser16

Specificity

Recognizes phosphorylated phospholamban (Ser16), Mr 5-25 kDa. Additional non-specific bands may be detected, Mr 75 & 100 kDa, in some lysates.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20C may cause glycerol containing solutions to become frozen during storage. Stable for 1 year at -20C from date of receipt.

Scientific Data Images for Phospholamban [p Ser16] Antibody - BSA Free

Phospholamban [p Ser16] Antibody

Western Blot-Phospholamban [p Ser16] Antibody-NBP3-18744-

Western Blot-Phospholamban [p Ser16] Antibody-NBP3-18744-Bovine cardiac preparation treated with PKA (lane1) or untreated (lane 2), was probed with anti-phospho-phospholamban (0.5 ug/ml).

Applications for Phospholamban [p Ser16] Antibody - BSA Free

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Western Blot: Routinely evaluated by western blot on bovine cardiac preparation that was incubated with PKA

0.5-2 ug/mL of this lot detected phosphorylated phospholamban from a bovine cardiac preparation that was incubated with 10 ug PKA for 10 minutes. DO NOT BOIL CARDIAC PREPARATION. Boiling may reduce the 25 kDa pentamer into tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, and 30% glycerol.

Format

BSA Free

Preservative

0.07% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phospholamban

Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1-25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25-52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.

Alternate Names

CMD1PPLBcardiac phospholamban, phospholamban

Gene Symbol

PLN

UniProt

P26678 (Human)

Additional Phospholamban Products

Product Documents for Phospholamban [p Ser16] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Phospholamban [p Ser16] Antibody - BSA Free

Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20C may cause glycerol containing solutions to become frozen during storage. Stable for 1 year at -20C from date of receipt.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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