Phospholipid Assay Kit (Colorimetric/Fluorometric)

Name: Phospholipid Assay Kit (Colorimetric/Fluorometric)
Brand: Novus Biologicals
SKU: KA1635
Price: 1089 USD
Availability: InStock

Novus Biologicals | Catalog # KA1635

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Product Documents

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Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Plasma (no EDTA), Serum (20 uL)

Sensitivity

OD, FL: 3, 0.6 uM

Assay Range

Colorimetric Assay: 3 to 200 uM, Fluorimetric Assay: 0.6 to 20 uM

View all Phospholipid Activity Assays »

Product Specifications

Assay Type

Colorimetric/Fluorometric

Kit Type

Assay Kit (Colorimetric/Fluorometric)

Species

Human, Mouse, Rat, Porcine, Yeast

Specificity

This kit can be used with both colorimetric and fluorometric detection systems.

Description

Quality control test: Standard Curve Phospholipid Assay Kit is a quantitative colorimetric/fluorimetric phospholipid determination.

Scientific Data Images for Phospholipid Assay Kit (Colorimetric/Fluorometric)

Phospholipid Assay Kit (Fluorometric) [KA1635] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Kit Contents for Phospholipid Assay Kit (Colorimetric/Fluorometric)

Assay Buffer
Dye Reagent
Enzyme Mix (Dried)
PLD Enzyme
Standard: 2 mM phosphatidylcholine

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Phospholipid

Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570 nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.

Additional Phospholipid Products

Product Documents for Phospholipid Assay Kit (Colorimetric/Fluorometric)

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Phospholipid Assay Kit (Colorimetric/Fluorometric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Citations for Phospholipid Assay Kit (Colorimetric/Fluorometric)

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