PIP5K2 gamma Overexpression Lysate

Name: PIP5K2 gamma Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-14433
Price: 689 USD
Availability: InStock

Novus Biologicals | Catalog # NBL1-14433

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for PIP5K2 gamma Overexpression Lysate

PIP5K2 gamma Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC205976

Accession#: NM_024779

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug) and 1 vial of empty vector negative control (100ug). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml).

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate.

Theoretical MW

47.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for PIP5K2 gamma Overexpression Lysate

Western Blot: PIP5K2 gamma Overexpression Lysate [NBL1-14433]

Western Blot: PIP5K2 gamma Overexpression Lysate (Adult Normal) [NBL1-14433] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PIP5K2 gamma.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: PIP5K2 gamma

PIP5K2 also known as Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma, has 3 isoforms, a 421 amino acid isoform that is 47 kDa, a 373 amino-acid isoform that is 42 kDa and a 403 amino-acid isoform that is 45 kDa; mostly found in the cytosol and surrounding plasma membrane; may be important in the production of Phosphatidylinositol bisphosphate (PIP2), in the endoplasmic reticulum. Disease research is currently being studied with relation to PIP5K2 and rheumatoid arthritis and arthritis. This protein has interactions with BTK, STK11, PIP4K2C, PIK3CG, PIK3R3, PIP5K1A, and over 40 other proteins in D-myo-inositol-5-phosphate metabolism, regulation of actin cytoskeleton, B cell Receptor signaling pathway, D-myo-inositol (1,4,5)-trisphosphate biosynthesis, superpathway of inositol phosphate compounds, inositol phosphate metabolism, and phosphatidylinositol signaling system pathways.

Alternate Names

EC 2.7.1, EC 2.7.1.149, FLJ22055, phosphatidylinositol-4-phosphate 5-kinase, type II, gamma, Phosphatidylinositol-5-phosphate 4-kinase type II gamma, phosphatidylinositol-5-phosphate 4-kinase type-2 gamma, phosphatidylinositol-5-phosphate 4-kinase, type II, gamma, PI(5)P 4-kinase type II gamma, PIP4KII-gamma, PIP5K2C

Gene Symbol

PIP4K2C

Additional PIP5K2 gamma Products

Product Documents for PIP5K2 gamma Overexpression Lysate

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Certificate of Analysis

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Product Specific Notices for PIP5K2 gamma Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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