PMSF

Tocris Bioscience | Catalog # 4486

Non-selective protease inhibitor; also blocks anandamide metabolism
Tocris Bioscience

Key Product Details

Description

Non-selective protease inhibitor; also blocks anandamide metabolism
View all Additional Synthetic Protease Inhibitors »

Product Description

PMSF is a non-selective protease inhibitor; irreversibly sulfonates key active site serine residues in serine proteases, acetylcholinesterases, and thiol proteases. Blocks metabolism of anandamide (Cat. No. 1339) in a rodent model of cannabimimetic activity. Unstable in water (t1/2 ~ 60 mins at pH 7.5). Brain penetrant.

Product Specifications for PMSF

Molecular Weight

174.19

Formula

C7H7FO2S

Storage

Desiccate at RT

Purity

≥98%

Chemical Name

Phenylmethylsulfonyl fluoride

CAS Number

329-98-6

PubChem ID

4784

InChI Key

YBYRMVIVWMBXKQ-UHFFFAOYSA-N

SMILES

O=S(CC1=CC=CC=C1)(F)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 17.42 100
Ethanol 17.42 100

Preparing Stock Solutions for PMSF

The following data is based on the product molecular weight 174.19.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.74 mL 28.70 mL 57.41 mL
5 mM 1.15 mL 5.74 mL 11.48 mL
10 mM 0.57 mL 2.87 mL 5.74 mL
50 mM 0.11 mL 0.57 mL 1.15 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 24 publications citing the usage of this product.

Product Documents for PMSF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PMSF

For research use only

Customer Reviews for PMSF (1)

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  • Works well for protein isolation
    Name: Nicole Brown
    Species: Mouse
    Assay Type: In Vivo
    Cell Line/Tissue: Kidney
    Verified Customer | Posted 01/07/2019
    Does not contain phosphatase inhibitors; add these to protease inhibitor mix if analyzing phosphorylated proteins
    Used to isolate protein from kidney tissue from wild type or IL-33 deficient mice that underwent cisplatin-induced kidney injury; several proteins were detected by immunoblot analysis; even after several freeze thaws, data has been consistent *additionally added a protease inhibitor cocktail and phosphatase inhibitor cocktail + RIPA for protein isolation
    PMSF 4486

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