POLR1B Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-93757

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all POLR1B Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 670-900 of human POLR1B (NP_061887.2). IANFIPFSDHNQSPRNMYQCQMGKQTMGFPLLTYQDRSDNKLYRLQTPQSPLVRPSMYDYYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKIKQGDSSLVFGIKPGDPRVLQKLDDDGLPFIGAKLQYGDPYYSYLNLNTGESFVMYYKSKENCVVDNIKVCSNDTGSGKFKCVCITMRVPRNPTIGDKFASRHGQKGILSRLWPAE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLR1B Antibody - Azide and BSA Free

Western Blot: POLR1B AntibodyAzide and BSA Free [NBP2-93757]

Western Blot: POLR1B AntibodyAzide and BSA Free [NBP2-93757]

Western Blot: POLR1B Antibody [NBP2-93757] - Analysis of extracts of various cell lines, using POLR1B at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for POLR1B Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POLR1B

POLR1B, also known as DNA-directed RNA polymerase I subunit RPA2, has a 1,135 amino acid long isoform that is approximately 128kDa, and a short 1,079 amino acid isoform that is approximately 121kDa. POLR1B is a RNA polymerase that is localized to the nucleolus. POLR1B is the second largest fundamental element of pol I, which is responsible for the production of ribosomal RNA. Current research on POLR1B is being performed in relation to several diseases and disorders including pneumonia, malaria and tuberculosis. POLR1B has also been shown to have interactions with Histone cluster 1 H4/A, H4/B, H4/C, H4/D and H4/E in pathways such as RNA Polymerase I transcription and RNA Polymerase III transcription.

Alternate Names

DNA-directed RNA polymerase I 135 kDa polypeptide, DNA-directed RNA polymerase I 135kDa polypeptide, DNA-directed RNA polymerase I subunit RPA2, EC 2.7.7.6, FLJ10816, FLJ21921, MGC131780, polymerase (RNA) I polypeptide B, 128kDa, RNA polymerase I subunit 2, RPA116, RPA135, RPA2, Rpo1-2

Gene Symbol

POLR1B

Additional POLR1B Products

Product Documents for POLR1B Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for POLR1B Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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