PP1 (Protein Phosphatase 1), also known as PPP1, is an enzyme that removes phosphate groups from serine or threonine residues. The holoenzyme is composed of two subunits. The catalytic subunit is highly conserved throughout evolution, and interacts with a wide range of proteins acting as regulatory subunits that target the enzyme to specific subcellular compartments and substrates. The catalytic subunit is ubiquitously expressed in tissues and cell lines.
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
DyLight 650 (Excitation = 652 nm, Emission = 672 nm)
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human PP1 alpha Catalytic Subunit
Lys41-Pro298
Accession # P62136
Lys41-Pro298
Accession # P62136
Specificity
Detects endogenous human, mouse and rat PP1 alpha in Western blots. Based on the immunogen’s homology, the antibody is expected to cross‑react with PP1 beta and PP1 gamma.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for PP1 Antibody [DyLight 650]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: PP1
Long Name
Protein Phosphatase 1
Alternate Names
PP1A;PP-1A;PP1alpha;PPP1A;PPP1CA;Serine/threonine-protein phosphatase;Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Gene Symbol
PPP1CA
Additional PP1 Products
Product Documents for PP1 Antibody [DyLight 650]
Product Specific Notices for PP1 Antibody [DyLight 650]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: Western Blot Figures
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- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars