Recombinant Human PPIH Protein

Novus Biologicals | Catalog # NBC1-18434

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Enzyme Activity, SDS-PAGE
View all PPIH Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-177 of Human PPIH

Source: E.coli

Amino Acid Sequence: MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

19.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 220 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PPIH Protein

SDS-PAGE: Recombinant Human PPIH Protein [NBC1-18434]

SDS-PAGE: Recombinant Human PPIH Protein [NBC1-18434]

SDS-Page: PPIH Protein [NBC1-18434] - Cyclophilin H(PPIH), 19.2 kDa (177 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation, and Storage

NBC1-18434
Formulation PBS (pH 7.4), 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPIH

Cyclophilin H (also known as peptidylpropyl isomerase H, PPIH) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. The cyclophilin H is a specific component of the human U4/U6 small nuclear ribonucleoprotein particle involved in the nuclear splicing of pre-mRNA. It stably associates with the U4/U6-60kD and -90kD proteins, the human orthologues of the Saccharomyces cerevisiae Prp4 and Prp3 splicing factors. Recombinant human cyclophilin H was expressed in E.coli and purified by conventional chromatography techniques.

Alternate Names

cyclophilin H, CYP20, CypH, CYPHpeptidyl prolyl isomerase H (cyclophilin H), EC 5.2.1.8, MGC5016, peptidyl-prolyl cis-trans isomerase H, peptidylprolyl isomerase H (cyclophilin H), PPIase H, Rotamase H, Small nuclear ribonucleoprotein particle-specific cyclophilin H, SnuCyp-20, USA-CyP SnuCyp-20, USA-CYPCYP-20, U-snRNP-associated cyclophilin SnuCyp-20, U-snRNP-associated cyclophilin SunCyp-20

Gene Symbol

PPIH

Additional PPIH Products

Product Documents for Recombinant Human PPIH Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human PPIH Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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