Protein A Antibody (1051739)
R&D Systems | Catalog # MAB99763
Protein A Monoclonal Antibody (Clone 1051739)
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Multi-Species
Applications
Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 1051739
Loading...
Product Specifications
Immunogen
Recombinant Protein A
Specificity
Detects Protein A in direct ELISA.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Protein A Antibody (1051739)
Detection of Multi-species Protein A by Western Blot.
Western blot shows recombinant Protein A Long, Protein A, Protein G (neg). PVDF membrane was probed with 2 µg/mL of Rat Anti-Protein A Monoclonal Antibody (Catalog # MAB99763) (Left) or an irrelevant Rat IgG (Right) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Protein A at approximately 55, 33 kDa (as indicated) only with MAB99763. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Multi-species Protein A by Simple WesternTM.
Simple Western lane view shows lysates of Protein A Long, Protein A, Protein G (negative control), loaded at 0.2 mg/mL. A specific band was detected for Protein A at approximately 40, 58 kDa (as indicated) using 20 µg/mL of Rat Anti-Protein A Monoclonal Antibody (Catalog # MAB99763) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Protein A Antibody (1051739)
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: Protein A Long, Protein A, Protein G (negative control)
Sample: Protein A Long, Protein A, Protein G (negative control)
Western Blot
2 µg/mL
Sample: Protein A Long, Protein A, Protein G (neg)
Sample: Protein A Long, Protein A, Protein G (neg)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Protein A
Additional Protein A Products
Product Documents for Protein A Antibody (1051739)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Protein A Antibody (1051739)
For research use only
Customer Reviews for Protein A Antibody (1051739)
There are currently no reviews for this product. Be the first to review Protein A Antibody (1051739) and earn rewards!
Have you used Protein A Antibody (1051739)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...