Recombinant Human PSMD13 His Protein

Novus Biologicals | Catalog # NBP2-51717

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all PSMD13 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-376 of Human PSMD13

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMKDVPGF LQQSQNSGPG QPAVWHRLEE LYTKKLWHQL TLQVLDFVQD PCFAQGDGLI KLYENFISEF EHRVNPLSLV EIILHVVRQM TDPNVALTFL EKTREKVKSS DEAVILCKTA IGALKLNIGD LQVTKETIED VEEMLNNLPG VTSVHSRFYD LSSKYYQTIG NHASYYKDAL RFLGCVDIKD LPVSEQQERA FTLGLAGLLG EGVFNFGELL MHPVLESLRN TDRQWLIDTL YAFNSGNVER FQTLKTAWGQ QPDLAANEAQ LLRKIQLLCL MEMTFTRPAN HRQLTFEEIA KSAKITVNEV ELLVMKALSV GLVKGSIDEV DKRVHMTWVQ PRVLDLQQIK GMKDRLEFWC TDVKSMEMLV EHQAHDILT

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

45.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PSMD13 His Protein

SDS-PAGE: Recombinant Human PSMD13 His Protein [NBP2-51717]

SDS-PAGE: Recombinant Human PSMD13 His Protein [NBP2-51717]

SDS-Page: Recombinant Human PSMD13 His Protein [NBP2-51717] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBP2-51717
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSMD13

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. [provided by RefSeq]

Alternate Names

HSPC027,26S proteasome regulatory subunit RPN9, p40.5,26S proteasome subunit p40.5,26S proteasome regulatory subunit p40.5, proteasome (prosome, macropain) 26S subunit, non-ATPase, 13,26S proteasome regulatory subunit S11, Rpn9,26S proteasome non-ATPase regulatory subunit 13, S11

Gene Symbol

PSMD13

Additional PSMD13 Products

Product Documents for Recombinant Human PSMD13 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human PSMD13 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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