PYR 41

Tocris Bioscience | Catalog # 2978

Ubiquitin-activating enzyme (E1) inhibitor
Tocris Bioscience

Key Product Details

Description

Ubiquitin-activating enzyme (E1) inhibitor
View all Ubiquitin-activating Enzyme (E1) Inhibitors »

Product Description

PYR 41 is a cell-permeable, irreversible ubiquitin-activating enzyme (E1) inhibitor (IC50 < 10 μM). Blocks ubiquitination and prevents ubiquitin-mediated proteasomal degradation. Inhibits NF-κB activation, blocks degradation of p53, increases p21 levels and induces apoptosis in vitro. Also causes an increase in sumoylation of proteins.

Product Specifications for PYR 41

Molecular Weight

371.30

Formula

C17H13N3O7

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

4-[4-[(5-Nitro-2-furanyl)methylene]-3,5-dioxo-1-pyrazolidinyl]benzoic acid ethyl ester

CAS Number

418805-02-4

PubChem ID

2856906

InChI Key

ARGIPZKQJGFSGQ-UHFFFAOYSA-N

SMILES

CCOC(=O)C1=CC=C(C=C1)N1NC(=O)C(=CC2=CC=C(O2)[N+]([O-])=O)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 37.13 100

Preparing Stock Solutions for PYR 41

The following data is based on the product molecular weight 371.30.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.69 mL 13.47 mL 26.93 mL
5 mM 0.54 mL 2.69 mL 5.39 mL
10 mM 0.27 mL 1.35 mL 2.69 mL
50 mM 0.05 mL 0.27 mL 0.54 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 21 publications citing the usage of this product.

Product Documents for PYR 41

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PYR 41

For research use only

Customer Reviews for PYR 41 (1)

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  • proteasomal degradation inhibitor
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Hepg2, Huh7
    Verified Customer | Posted 03/11/2020
    This compound worked very well for our experiments in 5 uM concentration.
    PYR 41 2978

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