Rad21 Antibody (9H0H6)

Novus Biologicals | Catalog # NBP3-15892

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9H0H6 expressed in HEK293
View all Rad21 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 532-631 of human Rad21 (O60216). EKEDDEEEEDEDASGGDQDQEERRWNKRTQQMLHGLQRALAKTGAESISLLELCRNTNRKQAAAKFYSFLVLKKQQAIELTQEEPYSDIIATPGPRFHII

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rad21 Antibody (9H0H6)

Western Blot: Rad21 Antibody (9H0H6) [NBP3-15892]

Western Blot: Rad21 Antibody (9H0H6) [NBP3-15892]

Western Blot: Rad21 Antibody (9H0H6) [NBP3-15892] - Western blot analysis of extracts of various cell lines, using Rad21 Rabbit mAb (NBP3-15892) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Rad21 Antibody (9H0H6)

Chromatin Immunoprecipitation: Rad21 Antibody (9H0H6) [Rad21] -

Chromatin Immunoprecipitation: Rad21 Antibody (9H0H6) [Rad21] - Chromatin immunoprecipitation analysis of extracts of A-549 cells, using Rad21 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Rad21 Antibody (9H0H6)

Chromatin Immunoprecipitation: Rad21 Antibody (9H0H6) [Rad21] -

Chromatin Immunoprecipitation: Rad21 Antibody (9H0H6) [Rad21] - Chromatin immunoprecipitation was performed with 10.7 ug of cross-linked chromatin from A-549 cells using 5 ug of Rad21 Rabbit mAb. DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2. The ChIP sequencing results indicate the enrichment pattern of Rad21 across chromosome 8 (upper panel) and the genomic region encompassing MYC, a representative gene enriched in Rad21 (lower panel).
Rad21 Antibody (9H0H6)

Chromatin Immunoprecipitation: Rad21 Antibody (9H0H6) [Rad21] -

Chromatin Immunoprecipitation: Rad21 Antibody (9H0H6) [Rad21] - Chromatin immunoprecipitation was performed with 10.7 ug of cross-linked chromatin from A-549 cells using 5 ug of Rad21 Rabbit mAb. DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2. The ChIP sequencing results indicate the enrichment pattern of Rad21 in the representative genomic region surrounding MYC gene.

Applications for Rad21 Antibody (9H0H6)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rad21

The cohesin complex plays an important role in tethering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys the cohesin complex by means of cleavaging its Scc1 subunit (Mcd1/Scc1/hRad21), allowing the chromatids to separate and divide with the cell. During apoptosis the C-terminal portion of hRad21 is cleaved and translocated to the cytoplasm. This cleavage and translocation may act as a nuclear signal that promotes and accelerates subsequent events of apoptosis (2).

Alternate Names

double-strand-break repair protein rad21 homolog, hHR21FLJ25655, HR21, KIAA0078RAD21 (S. pombe) homolog, MCD1, Nuclear matrix protein 1, NXP-1, NXP1HRAD21, protein involved in DNA double-strand break repair, RAD21 homolog (S. pombe), SCC1 homolog, SCC1FLJ40596

Gene Symbol

RAD21

Additional Rad21 Products

Product Documents for Rad21 Antibody (9H0H6)

Certificate of Analysis

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Product Specific Notices for Rad21 Antibody (9H0H6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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