Rat alpha 1-Microglobulin Alexa Fluor® 700-conjugated Antibody

Catalog #: AF7720N Datasheet / COA / SDS

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AF7720N-100UG

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Rat alpha 1-Microglobulin Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat alpha 1-Microglobulin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human alpha 1-Microglobulin is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat Î±1-Microglobulin
Asp20-Ala202
Accession # Q64240

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: alpha 1-Microglobulin

Rat alpha 1-Microglobulin (alpha1-m/A1M; also protein HC) is a secreted, 25-26 kDa glycoprotein member of the lipocalin family, calycin superfamily of molecules. It is expressed by hepatocytes, keratinocytes, and endodermal derivatives in the embryo. A1M appears to act as a heme scavenger, protecting cells and collagen against oxidative damage. It also acts as an immunosuppressant, inhibiting polyclonal lymphocyte activation and dampening granulocyte migration in response to chemokines. A1M circulates either as a monomer, or bound to IgA, albumin or prothrombin. Rat A1M is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140 kDa adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa A1M protein (aa 20-202), and a C-terminal serine protease inhibitor termed bikunin (aa 205-349). A1M possesses one lipocalin domain (aa 41-186). Although cleavage of AMBP in the Golgi apparatus typically generates a 25 kDa A1M and 28 kDa bikunin molecule, the 55-65 kDa AMBP precursor can also be released intact. In human, A1M will undergo extracellular processing, generating an isoform that is missing the C-terminal four amino acids. There are four potential isoform variants. One utilizes an alternative start site at Met181, a second contains an Asn substitution for aa 112-228, a third possesses an 11 aa substitution for aa 1-141, and a fourth shows a 17 aa substitution for aa 201-349. Over aa 20-202, rat A1M shares 76% and 86% aa sequence identity with human and mouse A1M, respectively.

Entrez Gene IDs

259 (Human); 11699 (Mouse); 25377 (Rat)

Alternate Names

A1M; alpha 1Microglobulin; alpha 1-Microglobulin; alpha-1-microglobulin/bikunin precursor; AMBP; bikunin; EDC1; HCP; HCPcomplex-forming glycoprotein heterogeneous in charge; HI30; IATIL; inter-alpha-trypsin inhibitor light chain; ITI; ITIL; ITILC; ITILgrowth-inhibiting protein 19; protein AMBP; protein HC; trypstatin; uristatin; uronic-acid-rich protein; UTI