Rat CXCL3/CINC-2 alpha / beta Antibody

Catalog #: AF517 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF517
AF517-SP

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Chemotaxis Induced by CXCL3/CINC‑2 beta and Neutral-ization by Rat CXCL3/ CINC‑2 alpha / beta Antibody.

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All CXCL3/GRO gamma/CINC-2/DCIP-1 Antibodies

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Rat CXCL3/CINC-2 alpha / beta Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CXCL3/CINC-2 alpha / beta in direct ELISAs and Western blots. In direct ELISAs, almost 100% cross-reactivity with recombinant rat (rr) CINC-2 alpha is observed, approximately 50% cross-reactivity with recombinant mouse (rm) MIP-2 is observed, 35% cross‑reactivity with rmKC is observed, 15% cross-reactivity with rrCINC-1 is observed, and 10% cross-reactivity with recombinant human (rh) IL‑8, rhGRO alpha, rhGRO beta, and rhGRO gamma is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat CXCL3/CINC-2 alpha / beta
Arg33-Leu100
Accession # NP_612531

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat CXCL3/CINC-2 alpha (Catalog # 516-CA)
Recombinant Rat CXCL3/CINC-2 beta (Catalog # 517-CB)

Neutralization

Measured by its ability to neutralize CXCL3/CINC-2 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.4-2.0 µg/mL in the presence of 0.05 µg/mL Recombinant Rat CXCL3/CINC-2 beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CXCL3/CINC‑2 beta and Neutral-ization by Rat CXCL3/ CINC‑2 alpha / beta Antibody. Recombinant Rat CXCL3/ CINC-2 beta (Catalog # 517-CB) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL3/ CINC-2 beta (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CXCL3/ CINC-2a/ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF517). The ND₅₀ is typically 0.4-2.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL3/GRO gamma/CINC-2/DCIP-1

CINC-2 alpha and -2 beta are mRNA splice variant isoforms that differ by only 3 amino acids at their amino-termini. These proteins are CXC chemokines most closely related to human GROs and mouse MIP-2. They are produced by granulation tissue, activated macrophages and fibroblasts. CINC-2 biological activities are mediated by CXCR1 or CXCR2.

Entrez Gene IDs

2921 (Human); 330122 (Mouse); 171551 (Rat)

Alternate Names

chemokine (C-X-C motif) ligand 3; CINC-2; CINC-2b; C-X-C motif chemokine 3; CXCL3; Dcip1; Gm1960; GRO gamma; GRO3 oncogene; GRO3; GROG; GRO-gamma; GRO-gamma(1-73); Growth-regulated protein gamma; Macrophage inflammatory protein 2-beta; melanoma growth stimulatory activity gamma; MGSA gamma; MIP2B; MIP-2b; MIP2-beta; SCYB3