Rat IL-12 Antibody

R&D Systems | Catalog # AF1760

R&D Systems

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-12 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat IL‑12
p40: Met23-Ser335; p35: Arg23-Ser215
Accession # p40: NP_072133; p35: Q9R103

Specificity

Detects rat IL-12 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IL‑12 and less than 5% cross-reactivity with recombinant human IL-12 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.30 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Rat IL-12 Antibody

Cell Proliferation Induced by IL‑12 and Neutralization by Rat IL‑12 Antibody.

Cell Proliferation Induced by IL‑12 and Neutralization by Rat IL‑12 Antibody.

Recombinant Rat IL-12 (Catalog # 1760-RL) stimulates proliferation in mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1760). The ND50 is typically 0.05-0.25 µg/mL.

Applications for Rat IL-12 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Rat IL‑12 (Catalog # 1760-RL)

Neutralization

Measured by its ability to neutralize IL‑12-induced proliferation in mouse splenocytes. The Neutralization Dose (ND50) is typically 0.05-0.25 µg/mL in the presence of 1 ng/mL Recombinant Rat IL‑12. This antibody will also neutralize rrIL-23 activity.

Reviewed Applications

Read 1 review rated 5 using AF1760 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-12

Interleukin 12 (IL-12) is the founding member of the IL-12 family of heterodimeric cytokines, which have important immunological functions. IL-12 is composed of two disulfide-linked subunits of 35 kDa and 40 kDa, respectively. The 35 kDa subunit (p35) is an alpha -helical protein homologous to IL-6 and G‑CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also utilized in IL-23. Mature rat p35 is a 194 amino acids (aa) protein that is secreted as a heterodimer linked to p40. It contains three potential N-linked glycosylation sites and shares 86%, and 58% aa sequence identity with mouse and human p35, respectively. Mature rat p40 contains 313 aa and can exist in multiple forms, including monomer, homodimer, heterodimer linked to p19 (forming IL-23), and heterodimer linked to p35 (forming IL-12). Mature rat p40 shows 92% and 66% aa sequence identity to mouse and human p40, respectively. Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. The activities of IL-12 are mediated by the receptor complex composed of two type I transmembrane proteins: a 100 kDa ligand-binding subunit (IL‑12 R beta 1) and a 130 kDa signal transducing subunit (IL‑12 R beta 2). IL‑12 facilitates hematopoietic stem cell proliferation, induces NK cell proliferation, and potentiates the expansion and late activation of Th1 CD4+ T cells (1-4).

References

  1. Park, A.Y. and P. Scott (2001) Scand. J. Immunol. 53:529.
  2. Trinchieri, G. et al. (2003) Immunity 19:641.
  3. Brombacher, F. et al. (2003) Trends Immunol. 24:207.
  4. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.

Long Name

Interleukin 12

Alternate Names

CLMF1, IL12, IL12A, NKSF1

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat); 397053 (Porcine); 403977 (Canine); 102115316 (Cynomolgus Monkey); 493741 (Feline); 703205 (Rhesus Macaque)

Gene Symbol

IL12A

UniProt

p40: NP_072133; p35: Q9R103

Additional IL-12 Products

Product Documents for Rat IL-12 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat IL-12 Antibody

For research use only

Citations for Rat IL-12 Antibody

Customer Reviews for Rat IL-12 Antibody (1)

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  • Rat IL-12 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Rat
    Verified Customer | Posted 11/08/2017
    This antibody was used to build an ELISA to measure rat IL-12. The antibody served well as both the capture and detection in a sandwich ELISA format. Rat Il-12 was measured in serum samples.
    Rat IL-12 Antibody AF1760

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