Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Neurexin 1 beta/NXRN1b Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Neurexin 1 beta /NXRN1b
Ala47-Thr392
Accession # Q63373

Specificity

Detects rat Neurexin 1 beta /NXRN1b in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant rat Neurexin 1 alpha is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Rat Neurexin 1 beta /NXRN1b Antibody

Detection of Rat Neurexin 1 beta /NXRN1b antibody by Western Blot.

Detection of Rat Neurexin 1 beta /NXRN1b by Western Blot.

Western blot shows lysates of rat spinal cord tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat Neurexin 1 beta /NXRN1b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4524) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurexin 1 beta /NXRN1b at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Rat Neurexin 1 beta /NXRN1b Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Rat spinal cord tissue

Reviewed Applications

Read 1 review rated 4 using AF4524 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Neurexin 1 beta/NXRN1b

Neurexin 1 beta is an 80 kDa member of the Neurexin family of proteins. It is expressed on neuronal presynaptic membranes and contributes to synapse formation through binding to postsynaptic neuroligins. An alternate start site in the Neurexin 1 gene generate an alpha - (long) and beta - (short) isoform. Mature rat Neurexin 1 beta is a type I transmembrane glycoprotein that is 468 amino acids (aa) in length. It has a 346 aa extracellular region (aa 47‑392) that contains one laminin-like G domain (aa 91‑264). Within the G domain, the presence or absence of a splice site (aa 201‑230) materially impacts neuroligin binding. Over aa 47‑392, rat Neurexin 1 beta is 100% and 98% aa identical to mouse and human Neurexin 1 beta, respectively.

Alternate Names

NXR1B, NXRN1b

Entrez Gene IDs

9378 (Human); 18189 (Mouse); 60391 (Rat)

Gene Symbol

NRXN1

UniProt

Q63373

Additional Neurexin 1 beta/NXRN1b Products

Product Documents for Rat Neurexin 1 beta /NXRN1b Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Rat Neurexin 1 beta /NXRN1b Antibody

For research use only

Citations for Rat Neurexin 1 beta /NXRN1b Antibody

Customer Reviews for Rat Neurexin 1 beta /NXRN1b Antibody (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Rat Neurexin 1 beta /NXRN1b Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 1 的 1 review Showing All
Filter By:
Clear Filters X
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 22840401
    Species: Rat
    Verified Customer | Posted 01/08/2015

There are no reviews that match your criteria.

Showing  1 - 1 的 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies