Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Oncomodulin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat Oncomodulin
Met1-Ser109
Accession # P02631

Specificity

Detects rat Oncomodulin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Oncomodulin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat Oncomodulin Antibody

Detection of Rat Oncomodulin antibody by Western Blot.

Detection of Rat Oncomodulin by Western Blot.

Western blot shows lysates of rat placenta tissue. PVDF Membrane was probed with 1 µg/mL of Rat Oncomodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6345) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Oncomodulin at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Rat Oncomodulin Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Rat placenta tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Oncomodulin

Oncomodulin (OM; also parvalbumin beta) is a 12 - 14 kDa member of the parvalbumin family of Ca++binding proteins. It is expressed in early embryonic cells, placenta, and in tumors. OM was originally thought to have expression restricted to neoplastic tissues, early embryonic cells and certain tumor cell lines. Recent research shows that oncomodulin is also expressed and secreted by macrophages where, in the retina, it binds to retinal ganglion cells (RGCs) and functions to promote axon regenerationin early embryonic cells, placenta, and in tumors. OM is both cytoplasmic, and secreted. Rat OM is 109 amino acids (aa) in length. It contains a vestigial Ca++binding site (aa 733) and two EF hand domains, the latter of which contains one highaffinity Ca++binding site (aa 78108). Relative to parvalbumin alpha, OM has a lower pI (<4.8), a higher affinity for Ca++, and they share only 50% aa identity. Full length rat OM shares 95% and 89% aa identity with mouse and human OM, respectively.

Alternate Names

OCM, OCM1, OCMN, OM, ONCM, Parvalbumin beta

Entrez Gene IDs

654231 (Human); 18261 (Mouse); 25503 (Rat)

Gene Symbol

OCM

UniProt

P02631

Additional Oncomodulin Products

Product Documents for Rat Oncomodulin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat Oncomodulin Antibody

For research use only

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